Developing molecular biology kits for more sensitive diagnostics and gene expression analysis

开发分子生物学试剂盒以实现更灵敏的诊断和基因表达分析

• 批准号: BB/J021601/1
• 负责人: Tamas Dalmay
• 金额: $ 15.28万
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ021601%2F1
• 关键词: Developing; molecular; biology; kits; more

Molecular biology kits are widely used for research and diagnostics and are a multibillion dollar industry. Many of these kits use enzymes that modify DNA and RNA. Improving the accuracy and sensitivity of these enzymes can provide a kit with the unique selling point. In BBSRC-funded research, we have developed a novel approach to study DNA or RNA modifying enzymes using Next Generation Sequencing (NGS). This rapid, low-cost method was used to identify factors that improve the activity of DNA polymerases used in PCR, and RNA ligases used in kits that capture small RNAs (sRNAs). We plan to develop a High-Definition (HD) method that more accurately captures RNA molecules, which have been linked with cancers and disease. Funding is also requested to develop a PCRmax technology to generate diagnostic kits with increased sensitivity. PCRmax technology is a rare opportunity to develop a method leading to a step change in PCR efficiency.

分子生物学试剂盒广泛用于研究和诊断，是一个数十亿美元的产业。这些试剂盒中的许多使用修饰DNA和RNA的酶。提高这些酶的准确性和灵敏度可以提供具有独特卖点的试剂盒。在BBSRC资助的研究中，我们开发了一种使用下一代测序（NGS）研究DNA或RNA修饰酶的新方法。这种快速，低成本的方法被用来识别提高PCR中使用的DNA聚合酶活性的因素，以及捕获小RNA（sRNA）的试剂盒中使用的RNA连接酶。我们计划开发一种高清（HD）方法，更准确地捕获与癌症和疾病有关的RNA分子。还要求提供资金，以开发最大聚合酶链反应技术，生产灵敏度更高的诊断试剂盒。PCRmax技术是开发导致PCR效率阶跃变化的方法的难得机会。

项目成果

Small RNA analysis in Sindbis virus infected human HEK293 cells.

• DOI: 10.1371/journal.pone.0084070
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Donaszi-Ivanov A;Mohorianu I;Dalmay T;Powell PP
• 通讯作者: Powell PP

Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.

• DOI: 10.1371/journal.pone.0103590
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Xu P;Mohorianu I;Yang L;Zhao H;Gao Z;Dalmay T
• 通讯作者: Dalmay T

Reducing ligation bias of small RNAs in libraries for next generation sequencing.

• DOI: 10.1186/1758-907x-3-4
• 发表时间: 2012-05-30
• 期刊: Silence
• 作者: Sorefan K;Pais H;Hall AE;Kozomara A;Griffiths-Jones S;Moulton V;Dalmay T
• 通讯作者: Dalmay T