Sensor Integrated Biosensors (SIBs)

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We propose to develop sensor integrated bioreactors (SIBs) as a tool enabling researchers and producers of tissueconstucts to monitor their development non-destructively during long-term culture (up to 28 days). The innovation in thisproposal is the incorporation of advanced sensing, imaging and monitoring technologies into an existing state-of-the-artbioreactor system: Quasi-Vivo. The added functionality will allow for non-invasive, in situ monitoring of tissue constructswhich will avoid the need to sacrifice experiments to collect data at each time point. The SIBs will be exploited by the endusergroup established in this consortium, both to speed the development cycle of regenerative medicine products andimprove the economics of production by providing a more closely controlled in-vitro model than is currently available.This project will deliver cell culture systems that provide faster growth of tissue, more accurate control of phenotype andimproved efficiency through the ability to monitor tissue development non-destructively in a flow-through system. Thesystem will be validated in a particular application for bone regeneration using RegenTec's ' injectable bone' scaffold.However the Quasi-Vivo bioreactors incorporating the Sensor Integrated Bioreactors (SIBs) technology developed in thisproject will have a wider range of application than just bone regeneration. The initial size of the bioreactor will be ideal forresearch applications but it has already been demonstrated that up to 36 Quasi-Vivo bioreactors can be operated in parallelmaking them inherently scaleable for stem cell propagation for clinical use.