A functional dissection of the bacterial replisome

细菌复制体的功能解剖

• 批准号: DP180100858
• 负责人: Prof Antoine van Oijen
• 金额: $ 36.75万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP180100858
• 关键词: functional; dissection; bacterial; replisome

This project aims to study the replisome, the machine that duplicates DNA before cell division. Years of biochemical research has shown how its protein components work, but observation at the single-molecule level is needed to understand how they all work together. This project aims to combine novel single-molecule biophysical tools with state-of-the-art biochemistry to define how the bacterial replisome coordinates synthesis of the two DNA strands and how it exchanges protein components on the fly. Expected outcomes of this project include improved understanding of a fundamental biological process, development of novel biophysical methodology, and training of the next generation of interdisciplinary scientists.

这个项目旨在研究复制体，这是一种在细胞分裂前复制DNA的机器。多年来的生化研究已经显示了它的蛋白质成分是如何工作的，但需要在单分子水平上进行观察，以了解它们是如何共同工作的。该项目旨在将新颖的单分子生物物理工具与最先进的生物化学相结合，以定义细菌复制体如何协调两条DNA链的合成，以及它如何在飞行中交换蛋白质成分。该项目的预期成果包括更好地理解基本的生物过程，开发新的生物物理学方法，以及培训下一代跨学科科学家。