Superresolution fluorescence imaging in microbiology

微生物学中的超分辨率荧光成像

• 批准号: LE160100127
• 负责人: Prof Antoine van Oijen
• 金额: $ 23.67万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE160100127
• 关键词: Superresolution; fluorescence; imaging; microbiology

Superresolution fluorescence imaging in microbiology: This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein function inside living cells. The new equipment is designed to provide a fast super-resolution imaging system to study the intracellular dynamics of proteins in vitro and a super-resolution microscope to visualise structures and assemblies inside microbes with a resolution of tens of nanometres, putting in vitro biochemistry into the context of a living cell.

微生物学中的超分辨率荧光成像： 该项目涉及购买新的和升级现有的荧光成像工具，以促进以比迄今为止可能的更高的空间和时间分辨率研究微生物系统中的细胞内过程。在最大分辨率下可视化细胞内分子组装的结构和动力学是了解活细胞内蛋白质功能所必需的。新设备旨在提供一个快速的超分辨率成像系统，以研究体外蛋白质的细胞内动力学，以及一个超分辨率显微镜，以数十纳米的分辨率可视化微生物内部的结构和组装，将体外生物化学置于活细胞的背景下。