Functional Dissection of the Bacterial Replisome

细菌复制体的功能解剖

• 批准号: DP150100956
• 负责人: Prof Antoine van Oijen
• 金额: $ 53.77万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150100956
• 关键词: Functional; Dissection; Bacterial; Replisome

This project aims to develop and use a suite of new single-molecule techniques to define how the bacterial replisome really works. The replisome is the machine that makes DNA in cells that are about to divide. Replisomes have many mechanistic challenges as they work to copy both strands of DNA at the same time. Many years of classic biochemical studies have worked out how many of these challenges are overcome. In recent years, the use of single-molecule biophysical techniques has begun to challenge many aspects of the elegant textbook view of replisome function. This approach is expected to reveal how synthesis of the two DNA strands in different directions at the same time is coupled together and how timing mechanisms work.

该项目旨在开发和使用一套新的单分子技术来定义细菌复制体的真正工作原理。复制体是在即将分裂的细胞中制造DNA的机器。当复制体同时复制两条DNA链时，它们面临着许多机制上的挑战。多年的经典生物化学研究已经计算出这些挑战中有多少是可以克服的。近年来，单分子生物物理技术的应用已经开始挑战教科书中关于复制体功能的许多方面。这种方法有望揭示两条DNA链在不同方向同时合成是如何耦合在一起的，以及定时机制是如何工作的。