MUSCLE DAMAGE IN AGING--INFLAMMATORY MECHANISMS

衰老过程中的肌肉损伤——炎症机制

• 批准号: 3159766
• 负责人: JOSEPH G CANNON
• 金额: $ 16.77万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1992-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3159766
• 关键词: aging; antiinflammatory agents; biomarker; biopsy; complement deficiency; cytotoxicity; exercise; histocompatibility antigens; histology; human old age (65+); human subject; human therapy evaluation; immune complex diseases; immunoregulation; indomethacin; inflammation; interleukin 1; muscle disorders; muscle proteins; omega 3 fatty acid; tumor necrosis factor alpha; young adult human (21-34)

Unaccustomed muscular activity of even moderate intensity results in a certain amount of damage to contractile proteins and associated connective tissue. Eccentric exercise, in which muscles lengthen as they develop tension, is a natural part of most forms of exercise and is a particularly potent inducer of damage. An inflammatory response is associated with this muscle damage which might contribute to catabolic processes and may mediate muscle protein turn over responsible for repair. We propose to use eccentric exercise to examine age-related differences in the inflammatory response associated with muscle damage. The inflammatory response will be measured in blood and in skeletal muscle. Circulating immune complexes, functional complement activity, interleukin-l (IL-l), humor necrosis factor (TNF), and humoral cytotoxic factors will be measured. In muscle, HLA class I antigens, IL-l, and TNF concentrations and receptor numbers will be determined and the immunohistological characterization of invading leukocytes will be performed. The influence of the non- steroidal anti-inflammatory drug indomethacin, and the influence of dietary omega-3 fatty acid supplementation on all of these parameters will be assessed as well. By examining the systemic and intramuscular processes involved in damaging exercise, the mechanisms which may be critically important effectors of repair and peripheral training in young and old subjects will be characterized for the first time. This study will bring together novel methods to examine responses to muscle damage and differentiate between the young and the old. These data will be important for the athlete, but perhaps more important for the millions of old and young people beginning a program of increased physical activity. Moreover, the eccentric exercise protocol may prove to be a safe and controlled alternative experimental model for studying inflammation the elderly.

不习惯的肌肉活动，即使是中等强度的结果 对收缩蛋白造成一定程度的损害， 相关结缔组织。 离心运动，其中肌肉 当它们产生张力时，它们会变长，这是大多数形体自然的一部分 是一种特别有效的损伤诱导剂。 一个 炎症反应与这种肌肉损伤有关， 可能有助于分解代谢过程， 负责修复的蛋白质周转。 我们建议使用 离心运动，以检查年龄相关的差异， 与肌肉损伤相关的炎症反应。 的 将在血液和骨骼中测量炎症反应 肌肉. 循环免疫复合物，功能性补体 活性、白细胞介素-I（IL-1）、体液坏死因子（TNF），和 将测量体液细胞毒性因子。 在肌肉中，HLA类 I抗原、IL-1和TNF浓度和受体数量将 免疫组织化学表征 将进行白细胞侵入。 非-- 甾体类抗炎药吲哚美辛及其影响 补充omega-3脂肪酸 参数也将进行评估。 通过检查系统和 肌内过程参与破坏性运动， 可能是修复的关键重要效应器的机制 在年轻人和老年人的科目的周边培训将是 第一次被描述。 这项研究将汇集 新的方法来检查对肌肉损伤的反应， 区分年轻人和老年人。 这些数据将 对运动员来说很重要，但对运动员来说更重要的是， 数以百万计的老年人和年轻人开始了一项增加 体力活动。 此外，偏心锻炼方案可 证明是一种安全可控的替代实验模型 研究老年人的炎症。