RADIOACTIVE LABELING OF BLOOD CELLS AND THEIR PRODUCTS

该项目旨在改进目前标记白细胞的方法。 和带有伽马发射剂的血小板，用于描绘病灶 闪烁引起的炎性损害、内皮损伤和血栓 相机成像。将开发新的放射性药物，包括 8-羟基喹啉的类似物、二硫化合物和 双缩氨基硫脲。这些亲脂性的螯合剂将被贴上标签 ~(99m)Tc、~(111)In、~(197)Hg、~(203)Pb或~(97)Ru。连续流动淋洗 带有特殊离心机转子的系统将用于采集白细胞 用甲草胺或斯特拉坦密度梯度离心法 用来采集血小板。分离的标记细胞的存活率将是 通过对实验动物的重新注射和细胞测量进行评估 在血流中恢复和存活。它们在演示中的功效 将在动物身上评估局部炎性病变和血管损伤 通过伽马相机成像和直接组织放射分析。

This project seeks to improve the current methods of labeling leukocytes and platelets with gamma-emitting agents for the delineation of focal inflammatory lesions, endothelial injury and thrombi by scintillation camera imaging. New radiopharmaceuticals will be developed, including analogues of 8-hydroxyquinoline, disulphide compounds and bis-thiosemicarbazones. These lipophilic chelating agents will be labeled with 99mTc, 111In, 197Hg, 203Pb or 97Ru. A continuous flow elutriation system with a special centrifuge rotor will be used to harvest leukocytes and centrifugation with Metrizamide or Stractan density gradients will be used to harvest platelets. Viability of the isolated labeled cells will be assessed by reinjection into experimental animals and measurement of cell recovery and survival in the blood stream. Their efficacy in demonstrating focal inflammatory lesions and vascular damage will be assessed in animals by gamma camera imaging and by direct tissue radioassay.