IMMUNOLOGIC STUDIES RELATED TO MALIGNANCY

与恶性肿瘤相关的免疫学研究

• 批准号: 3165035
• 负责人: CAROL A JONES
• 金额: $ 15.84万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-30 至 1990-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3165035
• 关键词: antibody formation; cell fusion; chromosomes; clone cells; genetic mapping; genetic markers; human tissue; hybrid cells; immunogenetics; monoclonal antibody; neoplasm /cancer immunology; neoplastic cell; surface antigens

We have developed a system to simplify the study of human cell surface structures by procedures which permit study of antigens due to individual chromosomes. A human-Chinese hameter, somatic cell hybrid, which contains chromosome 11 as its only human chromosome has been produced. This hybrid, which expresses human cell surface antigens, has been employed as a model system to study these components using a combined immunological, biochemical, and genetic approach. We have developed a deletion clone panel of chromosome 11, which has enabled us to conduct a variety of regional mapping studies including development of a new method using a low-repeat human DNA sequence. The production of monoclonal antibodies has allowed us to further characterize these antigens and to identify new antigens encoded by chromosome 11. We have begun experiments which should enable us to clone the genes for two of these antigens. We have recently carried out similar studies using hybrids that retain only human chromosomes 3 and 21. Deletion panels are being developed for these chromosomes and have been used for regional mapping. Studies of this kind should make possible rapid and definitive identification of a large variety of human cell surface antigens which may influence malignant behavior of human cells. The chromosomal location and biochemical identity of such genetic determinants may help elucidate genetic relationships of antigens involved in malignant response. (CS)

我们开发了一种系统来简化人体细胞表面的研究 通过允许研究个体抗原的程序进行结构 染色体。 人类与中国人的体细胞杂交体，其中含有 11号染色体作为其唯一的人类染色体已经产生。 这种混合体， 表达人类细胞表面抗原，已被用作模型 系统使用组合免疫学研究这些成分， 生物化学和遗传学方法。 我们开发了删除克隆 11 号染色体组，这使我们能够进行多种 区域测绘研究，包括开发一种使用 低重复人类 DNA 序列。 单克隆抗体的生产 使我们能够进一步表征这些抗原并识别新的 11号染色体编码的抗原。我们已经开始了实验，应该 使我们能够克隆其中两种抗原的基因。 我们最近进行了类似的研究，使用只保留 人类 3 号和 21 号染色体。正在为这些染色体开发删除面板 染色体并已用于区域绘图。 此类研究 应该能够快速、明确地鉴定大量品种 人类细胞表面抗原可能影响恶性行为 人体细胞。 此类物质的染色体位置和生化特性 遗传决定因素可能有助于阐明抗原的遗传关系 参与恶性反应。 （CS）