IMMUNOLOGIC STUDIES RELATED TO MALIGNANCY

我们已经开发了一种系统来简化对人类细胞表面的研究 允许研究由个体引起的抗原的程序的结构 染色体。一种人-中国汉密尔顿，体细胞杂交，它包含 11号染色体作为其唯一的人类染色体已经产生。这种混血儿， 它表达了人类细胞表面抗原，已经被用作模型 系统来研究这些成分使用组合的免疫学， 生物化学和遗传方法。我们已经开发出一种删除克隆 11号染色体的面板，它使我们能够进行各种 区域测绘研究，包括开发一种新方法，使用 低重复的人类DNA序列。克隆抗体的生产已经 使我们能够进一步表征这些抗原，并识别出新的 由11号染色体编码的抗原。我们已经开始了应该 使我们能够克隆其中两种抗原的基因。 我们最近进行了类似的研究，使用的是仅保留 人类3号和21号染色体。正在为这些项目开发删除面板 染色体，并已用于区域测绘。这类研究 应该能够快速和明确地识别大量的品种 人类细胞表面抗原可能会影响人的恶性行为 人类细胞。这类细胞的染色体定位和生化特性 遗传决定因素可能有助于阐明抗原的遗传关系 与恶性反应有关。(CS)

We have developed a system to simplify the study of human cell surface structures by procedures which permit study of antigens due to individual chromosomes. A human-Chinese hameter, somatic cell hybrid, which contains chromosome 11 as its only human chromosome has been produced. This hybrid, which expresses human cell surface antigens, has been employed as a model system to study these components using a combined immunological, biochemical, and genetic approach. We have developed a deletion clone panel of chromosome 11, which has enabled us to conduct a variety of regional mapping studies including development of a new method using a low-repeat human DNA sequence. The production of monoclonal antibodies has allowed us to further characterize these antigens and to identify new antigens encoded by chromosome 11. We have begun experiments which should enable us to clone the genes for two of these antigens. We have recently carried out similar studies using hybrids that retain only human chromosomes 3 and 21. Deletion panels are being developed for these chromosomes and have been used for regional mapping. Studies of this kind should make possible rapid and definitive identification of a large variety of human cell surface antigens which may influence malignant behavior of human cells. The chromosomal location and biochemical identity of such genetic determinants may help elucidate genetic relationships of antigens involved in malignant response. (CS)