IMMUNOLOGIC STUDIES RELATED TO MALIGNANCY

人类11号染色体的q23区域将用 利用体细胞杂交和人类细胞的免疫遗传学方法 由这些杂交种表达的表面抗原。单克隆抗体 识别这些抗原使它们可以作为选择性标记用于 操纵11号染色体区域的丢失和保留。 超过16种不同染色体亚型的白血病和淋巴瘤 11q23的重排，带有第二条但不同的染色体。一个 尤文肉瘤中的恶性相关易位涉及11q23。这个 最常见的经常性宪法移位也是在这一点上 染色体区域。 区域体细胞定位小组将细分11q23和脉冲场 凝胶电泳法将用于定位这些断裂点。隔离 断点及其序列的比较将用于测试 关于染色体重排机制的假说。这个 包括可能的原癌基因的基因序列的鉴定应该 增加了对恶性肿瘤发病机制的认识。

The q23 region of human chromosome 11 will be examined using an immunogenetic approach that utilizes somatic cell hybrids and human cell surface antigens expressed by these hybrids. Monoclonal antibodies recognizing these antigens allow them to be used as selective markers to manipulate the loss and retention of regions of chromosome 11. More than 16 different chromosomal subtypes of leukemia and lymphoma have a rearrangement of 11q23 with a second, but varying chromosome. A malignancy associated translocation in Ewing sarcoma involves 11q23. The most common recurrent constitutional translocation is also in this chromosomal region. Regional somatic cell mapping panels will subdivide 11q23 and pulsed field gel electrophoresis will be used to locate these breakpoints. Isolation of breakpoints and comparison of their sequences will be used to test hypotheses about mechanisms of chromosomal rearrangements. The identification of gene sequences including possible proto-oncogenes should add to the understanding of the pathogenesis of malignancy.