IMMUNOLOGIC STUDIES RELATED TO MALIGNANCY

与恶性肿瘤相关的免疫学研究

• 批准号: 3165041
• 负责人: CAROL A JONES
• 金额: $ 22.65万
• 依托单位: ELEANOR ROOSEVELT INST FOR CANCER RES
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3165041
• 关键词: chromosome deletion; chromosome translocation; gene rearrangement; genetic library; genetic mapping; genetic markers; genetic recombination; human tissue; hybrid cells; immunogenetics; laboratory mouse; monoclonal antibody; neoplasm /cancer genetics; neoplasm /cancer immunology; neoplasm /cancer relapse /recurrence; neoplastic cell; nephroblastoma; nucleic acid hybridization; nucleic acid sequence; protooncogene; pulsed field gel electrophoresis; surface antigens

The q23 region of human chromosome 11 will be examined using an immunogenetic approach that utilizes somatic cell hybrids and human cell surface antigens expressed by these hybrids. Monoclonal antibodies recognizing these antigens allow them to be used as selective markers to manipulate the loss and retention of regions of chromosome 11. More than 16 different chromosomal subtypes of leukemia and lymphoma have a rearrangement of 11q23 with a second, but varying chromosome. A malignancy associated translocation in Ewing sarcoma involves 11q23. The most common recurrent constitutional translocation is also in this chromosomal region. Regional somatic cell mapping panels will subdivide 11q23 and pulsed field gel electrophoresis will be used to locate these breakpoints. Isolation of breakpoints and comparison of their sequences will be used to test hypotheses about mechanisms of chromosomal rearrangements. The identification of gene sequences including possible proto-oncogenes should add to the understanding of the pathogenesis of malignancy.

人类11号染色体的q23区域将使用 利用体细胞杂交体和人细胞的免疫遗传学方法 这些杂交体表达的表面抗原。单克隆抗体 识别这些抗原允许它们被用作选择性标记， 操纵11号染色体区域的丢失和保留。 超过16种不同的白血病和淋巴瘤染色体亚型， 11q23与第二条不同染色体的重排。一 尤文肉瘤中恶性肿瘤相关易位涉及11q23。的 最常见的经常性体质易位也是在这个 染色体区域 区域体细胞定位板将细分11q23和脉冲场 凝胶电泳将用于定位这些断裂点。分离 断点及其序列的比较将用于测试 关于染色体重排机制的假说。的 包括可能的原癌基因在内的基因序列的鉴定应 增加对恶性肿瘤发病机制的了解。