HORMONAL REGULATION OF CULTURED ENDOMETRIAL CELLS

化学法培养兔子宫内膜上皮细胞 已开发出确定的培养基。 雌激素被发现促进细胞 分裂，而孕酮有相反的效果。 这些培养的 发现细胞是非循环（GO）或循环的。 两种细胞 亚群被分离，并且发现GO细胞是 雌激素的目标。孕酮与细胞周期的相互作用 是必要的，以获得对雌激素作用的拮抗作用， GO细胞由于扩散因子的产生。 类似的 一种可能是蛋白质的因子似乎是由高密度培养物产生的 对雌激素刺激没有反应。 在隔离的过程中 这种雌激素抑制因子，另一种具有雌激素放大作用的因子， 效果被发现。 还发现培养的细胞合成 一种叫做子宫珠蛋白的糖蛋白 它的生产受到以下因素的管制： 雌激素和孕酮。 雌二醇和孕酮减少了 雌激素受体在文化中，显然是通过不同的机制， 行动上 在漂浮胶原凝胶上生长的培养细胞表现出循环 生长动力学 这些细胞增殖和死亡的循环是由 由细胞产生的两种可扩散因子所控制。 这两个因素导致 无论是细胞死亡还是细胞增殖，显然都是蛋白质。 我们将 继续研究我们的实验系统，通过分析的机制， 卵巢性激素对细胞增殖的调节作用 死亡、子宫珠蛋白产生和激素受体，以及分离 调节细胞生长和雌激素作用的因素。 的端点 我们的研究是为了提高我们对性行为的机制的理解， 激素调节子宫内膜的增殖和分化。这是 我希望我们对电子邮件响应系统的研究将有助于 提高对子宫内膜异位症病因和发病机制的认识 特别是增生和癌，以及乳腺癌相关癌， 将军 （丁）

A technique to culture rabbit epithelial endometrial cells in chemically defined medium has been developed. Estrogens were found to promote cell division, whereas progesterone had an opposite effect. These cultured cells were found to be either noncycling (GO) or cycling. Both cell subpopulations were isolated, and it was found that the GO cells were the target of estrogens. Interaction between progesterone and the cycling cells was necessary to obtain an antagonistic effect on the estrogen action on the GO cells due to the production of a diffusible factor. A similar factor, probably a protein, appears to be produced by high density cultures that do not respond to estrogen stimulation. In the process of isolating this estrogen inhibitor factor, another one having estrogen-amplification effects was detected. It was also found that the cultured cells synthesize a glycoprotein called uteroglobin. Its production is regulated by estrogens and progesterone. Estradiol and progesterone decrease the number of estrogen receptors in cultures, apparently by different mechanisms of action. Cultured cells grown on floating collagen gels exhibit cycling growth kinetics. These cycles of cell proliferation and death are elicited by two diffusible factors made by the cells. These two factors inducing either cell death or cell proliferation, are apparently proteins. We will continue to study our experimental system by analyzing the mechanism of action of ovarian sex hormones in regulating cell proliferation, cell death, uteroglobin production, and hormone receptors, as well as isolating the factors regulating cell growth and estrogen effects. The endpoint of our research is to improve our comprehension of the mechanism by which sex hormones regulate proliferation and differentiation of endometrium. It\is expected that the study of our hormone-responsive system will help to advance the understanding of the etiology and pathogenesis of endometrial hyperplasia and carcinoma in particular and hormone-related cancer in general. (D)