UTEROGLOBIN EFFECTS AND MECHANISM OF ACTION

子宫珠蛋白的作用和作用机制

• 批准号: 3313599
• 负责人: LAZARO E GERSCHENSON
• 金额: $ 9.29万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-30 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3313599
• 关键词: autoradiography; cell cell interaction; cell growth regulation; cell population study; estrogens; gel filtration chromatography; globulins; growth inhibitors; progesterone; prostaglandin F; radioimmunoassay; radiotracer; scintillation spectrometry; tissue /cell culture

A technique to culture rabbit uterine epithelial cells in chemically defined medium has been developed. Estrogens were found to increase cell proliferation, while progesterone antogonized this effect. The primary cultures were found to be made up of quiescent or nondividing and dividing cells which show interactions. The cultures were also found to produce the rabbit uterus secretory protein uteroglobin. We have found prostaglandin F2Alpha and uteroglobin to be growth factors for our cultured cells. Preliminary evidence suggests that uteroglobin acts by increasing the amount of specific binding of prostglandin F2Alpha by the cells. The aims of this proposal are to eludidate the effects and mechanism of action of uteroglobin on rabbit endometriu. We will characterize further the prostaglandin F2Alpha receptor through kinetic studies, competition binding determinations and Scatchard analysis using cell membrane preparations obtained from endometrium scraped from rabbit uteri or cultures of rabbit endometrium epithelial cells. We will then examine the effect(s) of uteroglobin upon those parameters and the characteristics of prostaglandin binding in membranes isolated from endometrium from rabbits at different times after hCG injection, modulating in this way the uteroglobin concentration in uterine fluid. We will later determine the mechanism of uteroglobin interaction with rabbit endometrium through binding (after 125 Iodination), biochemical and cell proliferation studies, using cultured cells and whole animals. In summary, we believe that a uteroglobin-prostaglandin system plays an important role in endometrium biology, and maybe in other tissues. It is expected that our studies will help to increase the knowledge in the area of Reproductive Biology and Pathology, and by providing information on how hormones and local factors regulate proliferation and differentiation of endometrium, which are mechanisms implicated in implantation and decidualization.

兔子宫上皮细胞化学培养技术 已开发出确定的培养基。 发现雌激素增加细胞 而孕酮则拮抗这种作用。 主 发现培养物由静止或不分裂和分裂组成 显示相互作用的细胞。 还发现培养物产生 兔子宫分泌蛋白子宫珠蛋白。 我们发现前列腺素 F2 α和子宫珠蛋白是我们培养细胞的生长因子。 初步证据表明，子宫珠蛋白的作用是通过增加 细胞对前列腺素F2 α的特异性结合量。 目标 这一建议的目的是逃避的影响和作用机制， 子宫珠蛋白对家兔子宫的影响。 我们将进一步描述 前列腺素F2 α受体通过动力学研究，竞争结合 使用细胞膜制备物的测定和Scatchard分析 从兔子宫刮取的子宫内膜或兔培养物中获得 子宫内膜上皮细胞 然后我们将检查的影响（S） 子宫珠蛋白对这些参数和前列腺素特征的影响 不同温度下兔子宫内膜细胞膜的结合力 hCG注射后，以这种方式调节子宫珠蛋白 子宫液浓度。 我们稍后将确定 子宫珠蛋白通过结合与兔子宫内膜相互作用（125 碘化），生物化学和细胞增殖研究，使用培养的 细胞和整个动物。 总之，我们认为， 子宫球蛋白-前列腺素系统在子宫内膜中起重要作用 在生物学上，也许在其他组织中。 预计我们的研究将 帮助增加生殖生物学领域的知识， 病理学，并通过提供信息，如何激素和当地因素 调节子宫内膜的增殖和分化， 与着床和蜕膜化有关的机制。