UTEROGLOBIN EFFECTS AND MECHANISM OF ACTION

兔子宫上皮细胞的化学培养技术 已开发出定义介质。雌激素被发现可以增加细胞 细胞增殖，而黄体酮对此作用起安慰剂作用。初级阶段 文化被发现是由静止的或不分裂的和分裂的 显示相互作用的细胞。这些培养物也被发现产生了 兔子宫分泌蛋白，子宫红蛋白。我们发现了前列腺素 F2pha和子宫红蛋白是我们培养细胞的生长因子。 初步证据表明，子宫红蛋白的作用是通过增加 细胞与前列腺素F2pha的特异性结合量。目标 这一建议的目的是为了揭示 兔子宫内膜上的子宫红蛋白。我们将进一步描述 前列腺素F2α受体的动力学研究、竞争结合 利用细胞膜制剂进行测定和Scatchard分析 取自兔子宫或培养的兔子宫内膜 子宫内膜上皮细胞。然后我们再考察一下(S)的效果 子宫球蛋白对上述参数的影响及前列腺素的特性 不同时间兔子宫内膜膜的结合 人绒毛膜促性腺激素注射后，以这种方式调节子宫红蛋白 宫腔积液中的浓度。我们将在稍后确定其机制 子宫红蛋白通过结合与兔子宫内膜相互作用(125后 碘化)，生化和细胞增殖研究，使用培养的 细胞和整个动物。总而言之，我们认为 子宫球蛋白-前列腺素系统在子宫内膜中的重要作用 生物学，也许还存在于其他组织中。预计我们的研究将会 帮助增加生殖生物学领域的知识和 病理学，并提供关于激素和局部因素如何 调节子宫内膜的增殖和分化，它们是 涉及着床和蜕膜形成的机制。

A technique to culture rabbit uterine epithelial cells in chemically defined medium has been developed. Estrogens were found to increase cell proliferation, while progesterone antogonized this effect. The primary cultures were found to be made up of quiescent or nondividing and dividing cells which show interactions. The cultures were also found to produce the rabbit uterus secretory protein uteroglobin. We have found prostaglandin F2Alpha and uteroglobin to be growth factors for our cultured cells. Preliminary evidence suggests that uteroglobin acts by increasing the amount of specific binding of prostglandin F2Alpha by the cells. The aims of this proposal are to eludidate the effects and mechanism of action of uteroglobin on rabbit endometriu. We will characterize further the prostaglandin F2Alpha receptor through kinetic studies, competition binding determinations and Scatchard analysis using cell membrane preparations obtained from endometrium scraped from rabbit uteri or cultures of rabbit endometrium epithelial cells. We will then examine the effect(s) of uteroglobin upon those parameters and the characteristics of prostaglandin binding in membranes isolated from endometrium from rabbits at different times after hCG injection, modulating in this way the uteroglobin concentration in uterine fluid. We will later determine the mechanism of uteroglobin interaction with rabbit endometrium through binding (after 125 Iodination), biochemical and cell proliferation studies, using cultured cells and whole animals. In summary, we believe that a uteroglobin-prostaglandin system plays an important role in endometrium biology, and maybe in other tissues. It is expected that our studies will help to increase the knowledge in the area of Reproductive Biology and Pathology, and by providing information on how hormones and local factors regulate proliferation and differentiation of endometrium, which are mechanisms implicated in implantation and decidualization.