AVIAN SARCOMA VIRUS SPECIFIC TUMOR ANTIGENS

禽肉瘤病毒特异性肿瘤抗原

• 批准号: 3168592
• 负责人: J THOMAS PARSONS
• 金额: $ 14.58万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-01-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3168592
• 关键词: antiserum; autoradiography; avian sarcoma virus; cell growth regulation; genetic manipulation; genetic mapping; hybridomas; immunochemistry; immunogenetics; molecular oncology; monoclonal antibody; oncogenic virus; protein tyrosine kinase; radioimmunoassay; temperature sensitive mutant; tissue /cell culture; tumor antigens; viral carcinogenesis

Genetic and biochemical experiments have shown that the product of the Rous sarcoma virus src gene is required for the initiation and maintenance of cellular transformation. As a part of our long-term goal of understanding how the src gene product, pp60src, mediates the molecular events involved in cellular transformation, we have isolated and partially characterized monoclonal antibodies directed against pp60src. The experiments outlined in this proposal employ monoclonal antibodies to unique determinants within pp60src as immunochemical probes to define and characterize the structural and functional domains of the molecule and attempt to elucidate the role of individual functional domains in mediating cellular transformation. We further propose to utilize monoclonal antibodies to pp60src as immunochemical probes to compare the structure and function of other retrovirus tyrosine protein kinase as well as normal cell tyrosine protein kinases. These studies will permit us to define the structural and functional domains of pp60src and allow us to relate this information to other proteins which may be members of a family of related tyrosine protein kinases.

遗传和生物化学实验均表明，该产物为劳斯