SEQUENTIAL ANALYSIS OF ORAL CARCINOGENESIS

口腔癌发生的序贯分析

• 批准号: 3171930
• 负责人: Dennis B. Solt
• 金额: $ 13.11万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-07-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171930
• 关键词: angiogenesis; autologous transplantation; benzanthracenes; cellular oncology; chemical carcinogenesis; gastrointestinal epithelium; hamsters; histochemistry /cytochemistry; immunochemistry; keratinocyte; laboratory rat; microscopy; monoclonal antibody; mouth neoplasms; neoplasm /cancer genetics; neoplasm /cancer transplantation; nutrition related tag; oncogenes; oral pharyngeal neoplasm; preneoplastic state; tissue /cell preparation; tobacco; transforming growth factors; tumor promoters

The long-range objective of the proposed research is to identify and analyze in sequential fashion, the key cellular and molecular events occurring and during oral carcinogenesis. Viable cell dissociates, full thickness whole mounts, and cryostatprepared microscope sections, of carcinogen-treated hamster buccal pouch epithelium (HBPE) will be examined for functional, histochemical, immunohistochemical, and morphological evidence of cells which have undergone one or more steps in the carcinogenic Process. The specific aims are: 1) to (a) further characterize a unique keratinocyte population identified in cultures of carcinogen- initiated HBPE, and (b) evaluate its neoplastic potential using assays for production of transforming growth factors, in vitro growth characteristics, angiogenesis, and tumor formation following reimplantation into the autologous host, 2) to detect presumptive precancerous lesions in carcinogen-initiated HBPE using monoclonal antibodies against specific oncogene products, and 3) to determine whether extractable constituents of smokeless tobacco exert a promoting action on HBPE with an accompanying selection of focal cell populations rich in gamma-glutamyl transpeptidase activity. This research is based on the premise that a detailed analysis of oral carcinogenesis, which focuses on the relevant participating cell populations, will contribute to improved strategies for early detection of precancerous oral lesions and prevention of oral cancer.

拟议研究的长期目标是确定 并以连续的方式分析关键的细胞和分子 口腔癌发生过程中发生的事件。 活细胞 解离、全厚度全封片和制备的低温恒温器 显微镜切片，致癌剂处理的仓鼠颊囊 将检查上皮（HBPE）的功能、组织化学 免疫组织化学和细胞的形态学证据， 在致癌过程中经历了一个或多个步骤。 的 具体目标是：1）（a）进一步表征一个独特的 在致癌物培养物中鉴定的角质形成细胞群- 启动HBPE，和（B）使用 体外转化生长因子生产测定法 生长特性、血管生成和肿瘤形成 再植入自体宿主，2）检测推定的 使用单克隆抗体检测致癌物引发的HBPE中的癌前病变 针对特定癌基因产物的抗体，以及3）确定 无烟烟草的可提取成分是否发挥 促进对HBPE采取行动，同时选择重点 富含γ-谷氨酰转肽酶活性的细胞群。 本研究的前提是， 口腔癌的发生，其中重点是有关参与 细胞群，将有助于改善战略， 口腔癌前病变的检测和口腔癌的预防 癌