IN VITRO ANALYSIS OF ANTIBODY REGULATION IN HUMANS

人类抗体调节的体外分析

• 批准号: 3172714
• 负责人: RONALD H STEVENS
• 金额: $ 7.11万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172714
• 关键词: B lymphocyte; antibody formation; cell population study; clone cells; electrofocusing; helper T lymphocyte; humoral immunity; immunization; immunofluorescence technique; immunopharmacology; immunoregulation; leukocyte activation /transformation; plaque assay; radioimmunoassay; radiotracer; suppressor T lymphocyte; tetanus toxoid

Human lymphoblastoid B cells are a subset of highly activated B cells which are induced by in vivo immunization. In vitro these B cells produce antibody without the need for T-cell help or mitogen stimulation, and the majority of the antibody production is complete by day 3 of culture. In the past year, we found that the antibody-producing capacity of these cells can be reduced in vitro by NK cells and a separate suppressor T-cell subset. In the coming year, we will determine whether all NK cells have this inhibitory capacity or, as suggested by our earlier studies, only a subset of NK cells performs this function. We will test this by selective removal and/or isolation of different subsets of NK cells using monoclonal antibodies with differing specificities. We have recently determined that the two suppressor cells recognize different target structures on the B cell, with the NK cell recognizing the transferrin receptor and the T cell recognizing a separate, as yet undefined structure. It is also clear from our studies that not all lymphoblastoid B cells are capable of being inhibited. Although the two suppressor cells recognize different target structures, they appear to recognize the same B-cell subsets. This B-cell stage appears to be a proliferating cell which has not yet developed the capacity for antibody secretion. (LB)

人淋巴母细胞样B细胞是高度活化的B细胞的一个亚群

项目成果

12-O-Tetradecanoylphorbol-13-acetate (TPA) directly inhibits spontaneous immunoglobulin secretion by in vivo antigen-induced human lymphoblastoid B cells.

12-O-Tetradecanoylphorbol-13-acetate (TPA) 直接抑制体内抗原诱导的人淋巴母细胞 B 细胞的自发免疫球蛋白分泌。

• DOI: 10.1007/bf00915295
• 发表时间: 1984
• 期刊: Journal of clinical immunology
• 影响因子: 9.1
• 作者: Brieva,JA;Louie,JS;Stevens,RH
• 通讯作者: Stevens,RH

Involvement of the transferrin receptor in the production and NK-induced suppression of human antibody synthesis.

转铁蛋白受体参与产生和 NK 诱导的人类抗体合成抑制。

• 发表时间: 1984
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Brieva,JA;Stevens,RH
• 通讯作者: Stevens,RH

Clonally restricted anti-IgG antibodies in rheumatoid arthritis.

类风湿性关节炎中克隆限制性抗 IgG 抗体。

• DOI: 10.1002/art.1780271208
• 发表时间: 1984
• 期刊: Arthritis and rheumatism
• 作者: Persselin,JE;Louie,JS;Stevens,RH
• 通讯作者: Stevens,RH

Subclasses of human IgG anti-Fab antibodies: parameters for optimum detection.

人 IgG 抗 Fab 抗体的亚类：最佳检测参数。

• DOI: 10.1159/000233915
• 发表时间: 1985
• 期刊: International archives of allergy and applied immunology
• 作者: Persselin,JE;Keld,B;Fried,L;Stevens,RH
• 通讯作者: Stevens,RH

Human in vivo antigen-induced lymphoblastoid B cells are capable of cyclical antibody production in vitro.

人体内抗原诱导的类淋巴母细胞 B 细胞能够在体外周期性产生抗体。

• 发表时间: 1984
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Brieva,JA;Stevens,RH
• 通讯作者: Stevens,RH