MOLECULES MEDIATING LYMPHOCYTE ATTACHMENT

介导淋巴细胞附着的分子

• 批准号: 3176449
• 负责人: MICHAEL D PIERSCHBACHER
• 金额: $ 18.25万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-15 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176449
• 关键词: T lymphocyte; affinity chromatography; antibody formation; bone marrow; cell adhesion; cell cell interaction; cell differentiation; cell migration; cellular immunity; chemical structure function; density gradient ultracentrifugation; enzyme linked immunosorbent assay; human subject; immunofluorescence technique; lymphatic tissue; lymphoma; macromolecule; neoplastic cell; radiotracer; thymus; tissue /cell culture

Preliminary data are presented which demonstrate the attachment of lymphoma cells to attachment-promoting molecules such as fibronectin and serum spreading factor. Data are also discussed which indicate that normal lymphocytes from the thymus interact with at least one of these molecules--serum spreading factor. It is proposed here to study the interactions of lymphoid cells with known adhesion molecules. A plan is also presented for the identification of novel molecules involved in mediating the attachment of normal lymphocytes. The fact that normal bone marrow cells attach to a monolayer of thymic epithelium indicates that such molecules exist. These molecules will be examined for structure, function, and tissue distribution. The hypothesis is that the selective attachment of immature lymphocytes to molecules within the thymus probably modulates their differentiation, path of migration, and response to external stimuli. Understanding these interactions will give insight into the behavior and development of lymphocytes as well as possibly provide a better understanding of the behavior of lymphoid tumors. New methods which involve the study of cell attachment to electrophoretically separated proteins are proposed for studying this problem. (LB)

提供的初步数据显示淋巴瘤的附着。 细胞对纤维连接蛋白和血清等促进黏附分子的作用 扩散系数。还讨论了数据，这些数据表明正常 胸腺中的淋巴细胞至少与其中之一相互作用。 分子--血清传播因子。建议在这里研究一下 淋巴细胞与已知黏附分子的相互作用。计划是 也被用于鉴定涉及到的新分子 调节正常淋巴细胞的附着。事实上，正常的骨骼 骨髓细胞附着在胸腺上皮的单层表明 分子确实存在。这些分子将被检查结构，功能， 和组织分布。假设是选择性依恋 未成熟淋巴细胞对胸腺内分子的调节作用 他们的分化、迁移路径和对外部环境的反应 刺激物。了解这些交互作用将使我们深入了解 淋巴细胞的行为和发育以及可能提供一种 更好地了解淋巴样肿瘤的行为。新的方法， 涉及细胞与电泳法分离的细胞附着的研究 蛋白质被提出用来研究这一问题。(磅)