MOLECULES MEDIATING LYMPHOCYTE ATTACHMENT

介导淋巴细胞附着的分子

• 批准号: 3176454
• 负责人: MICHAEL D PIERSCHBACHER
• 金额: $ 17.35万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176454
• 关键词: T lymphocyte; affinity chromatography; antibody formation; bone marrow; cell adhesion; cell cell interaction; cell differentiation; cell migration; cellular immunity; chemical structure function; collagen; density gradient ultracentrifugation; enzyme linked immunosorbent assay; fibronectins; human subject; immunofluorescence technique; lymphatic tissue; lymphoma; macromolecule; neoplastic cell; radiotracer; thymus; tissue /cell culture

Preliminary data are presented which demonstrate the attachment of lymphoma cells to attachment-promoting molecules such as fibronectin and serum spreading factor. Data are also discussed which indicate that normal lymphocytes from the thymus interact with at least one of these molecules--serum spreading factor. It is proposed here to study the interactions of lymphoid cells with known adhesion molecules. A plan is also presented for the identification of novel molecules involved in mediating the attachment of normal lymphocytes. The fact that normal bone marrow cells attach to a monolayer of thymic epithelium indicates that such molecules exist. These molecules will be examined for structure, function, and tissue distribution. The hypothesis is that the selective attachment of immature lymphocytes to molecules within the thymus probably modulates their differentiation, path of migration, and response to external stimuli. Understanding these interactions will give insight into the behavior and development of lymphocytes as well as possibly provide a better understanding of the behavior of lymphoid tumors. New methods which involve the study of cell attachment to electrophoretically separated proteins are proposed for studying this problem. (LB)

初步资料显示淋巴瘤的附着