MOLECULES MEDIATING THE ATTACHMENT OF LYMPHOCYTES

介导淋巴细胞附着的分子

• 批准号: 3176452
• 负责人: MICHAEL D PIERSCHBACHER
• 金额: $ 12.83万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-15 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176452
• 关键词: T lymphocyte; affinity chromatography; antibody formation; bone marrow; cell adhesion; cell cell interaction; cell differentiation; cell migration; cellular immunity; chemical structure function; density gradient ultracentrifugation; enzyme linked immunosorbent assay; human subject; immunofluorescence technique; lymphatic tissue; lymphoma; macromolecule; neoplastic cell; radiotracer; thymus; tissue /cell culture

Preliminary data are presented which demonstrate the attachment of lymphoma cells to attachment-promoting molecules such as fibronectin and serum spreading factor. Data are also discussed which indicate that normal lymphocytes from the thymus interact with at least one of these molecules--serum spreading factor. It is proposed here to study the interactions of lymphoid cells with known adhesion molecules. A plan is also presented for the identification of novel molecules involved in mediating the attachment of normal lymphocytes. The fact that normal bone marrow cells attach to a monolayer of thymic epithelium indicates that such molecules exist. These molecules will be examined for structure, function, and tissue distribution. The hypothesis is that the selective attachment of immature lymphocytes to molecules within the thymus probably modulates their differentiation, path of migration, and response to external stimuli. Understanding these interactions will give insight into the behavior and development of lymphocytes as well as possibly provide a better understanding of the behavior of lymphoid tumors. New methods which involve the study of cell attachment to electrophoretically separated proteins are proposed for studying this problem. (LB)

初步数据显示，淋巴瘤的附件 细胞与促进附着的分子如纤连蛋白和血清 扩展因子 数据也进行了讨论，表明正常 来自胸腺的淋巴细胞与这些细胞中的至少一种相互作用， 分子血清扩散因子 这里建议研究 淋巴细胞与已知粘附分子的相互作用。 一项计划 也提出了新的分子参与识别 介导正常淋巴细胞的附着。 正常的骨骼 骨髓细胞附着在胸腺上皮细胞单层上表明， 分子存在。 这些分子的结构、功能， 和组织分布。 假设是选择性依恋 未成熟淋巴细胞与胸腺内分子的结合可能会调节 他们的分化、迁移路径以及对外部环境的反应 刺激。 了解这些相互作用将有助于深入了解 淋巴细胞的行为和发育，以及可能提供一个 更好地了解淋巴瘤的行为。 新方法， 包括研究细胞附着在分离的 蛋白质被提议用于研究这个问题。 （LB）