RECOMBINANT DNA DIAGNOSTIC PROBES FOR SMALL CELL CANCERS

小细胞癌重组 DNA 诊断探针

• 批准号: 3181283
• 负责人: WINSTON SALSER
• 金额: $ 11.16万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3181283
• 关键词: Ewing's tumor; cell differentiation; cell type; complementary DNA; gel electrophoresis; genetic manipulation; hematopoiesis; human subject; leukemia; lymphocytic leukemia; messenger RNA; molecular cloning; molecular pathology; myelogenous leukemia; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplastic cell; neuroblastoma; oncogenes; rhabdomyosarcoma; small cell lung cancer

We will use recombinant DNA technology to select cDNA clones that can improve diagnosis of small cell tumors. These cancers include oat cell carcinoma, many of the non-Hodgkin's lymphomas, Ewing's sarcoma, small cell tumors of neuroectodermal origin, embryonal rhabdomyosarcoma, and the leukemias. In many cases the tumor cells are primitive cells which may be difficult to classify because there is no evidence of features of maturation. Yet proper classification is important since treatment is often different depending upon the cell type of the tissue of origin for the malignant cells. It is now possible to use recombinant DNA techniques to create hybridization probes, which can assay mRNA species present in one cell type and not another. In theory, immunodiagnostic approaches also are capable of assaying any gene product in which two malignant cell types may differ, but only recombinant DNA techniques offer the ability to efficiently select the crucial gene product even if it is a rare mRNA species unique to only one of the two cell types. In earlier work we selected a panel of clones corresponding to abundant mRNAs strongly regulated during myeloid differentiation. These clones will be useful in distinguishing between myeloid and lymphoid cells. We expect that some members of this panel of clones may also be useful in distinguishing any hematopoietic cell from cells of other lineages. We will use the new hybridization-subtraction recombinant DNA technology to select additional cDNA clones which can improve the differential diagnosis of the small cell tumors. This approach, if successful, will improve subclassification of other cancer cell types so that the most effective treatment can be selected. (2)

我们将使用重组DNA技术来选择cDNA克隆

项目成果

Use of deoxyribonucleic acid probes in the identification of cell origin and detection of cellular contamination in human lymphoblastoid cell lines.

使用脱氧核糖核酸探针鉴定人淋巴母细胞系的细胞起源和检测细胞污染。

• 发表时间: 1989
• 期刊: Laboratory investigation; a journal of technical methods and pathology
• 作者: Naeim,F;Jensen,L;Susi,E;Yam,P;Gatti,R;Salser,W;Concannon,P
• 通讯作者: Concannon,P

Informativeness of VNTR genetic markers for detecting chimerism after bone marrow transplantation.

VNTR 遗传标记用于检测骨髓移植后嵌合状态的信息。

• 发表时间: 1989
• 期刊: Disease markers
• 作者: Gatti,RA;Nakamura,Y;Nussmeier,M;Susi,E;Shan,W;Grody,WW
• 通讯作者: Grody,WW