RECOMBINANT DNA DIAGNOSTIC PROBES FOR SMALL CELL CANCERS
小细胞癌重组 DNA 诊断探针
基本信息
- 批准号:3181283
- 负责人:
- 金额:$ 11.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1985
- 资助国家:美国
- 起止时间:1985-09-30 至 1989-07-31
- 项目状态:已结题
- 来源:
- 关键词:Ewing's tumor cell differentiation cell type complementary DNA gel electrophoresis genetic manipulation hematopoiesis human subject leukemia lymphocytic leukemia messenger RNA molecular cloning molecular pathology myelogenous leukemia neoplasm /cancer classification /staging neoplasm /cancer diagnosis neoplastic cell neuroblastoma oncogenes rhabdomyosarcoma small cell lung cancer
项目摘要
We will use recombinant DNA technology to select cDNA clones that can
improve diagnosis of small cell tumors. These cancers include oat cell
carcinoma, many of the non-Hodgkin's lymphomas, Ewing's sarcoma, small cell
tumors of neuroectodermal origin, embryonal rhabdomyosarcoma, and the
leukemias.
In many cases the tumor cells are primitive cells which may be difficult to
classify because there is no evidence of features of maturation. Yet
proper classification is important since treatment is often different
depending upon the cell type of the tissue of origin for the malignant
cells.
It is now possible to use recombinant DNA techniques to create
hybridization probes, which can assay mRNA species present in one cell type
and not another. In theory, immunodiagnostic approaches also are capable
of assaying any gene product in which two malignant cell types may differ,
but only recombinant DNA techniques offer the ability to efficiently select
the crucial gene product even if it is a rare mRNA species unique to only
one of the two cell types.
In earlier work we selected a panel of clones corresponding to abundant
mRNAs strongly regulated during myeloid differentiation. These clones will
be useful in distinguishing between myeloid and lymphoid cells. We expect
that some members of this panel of clones may also be useful in
distinguishing any hematopoietic cell from cells of other lineages.
We will use the new hybridization-subtraction recombinant DNA technology to
select additional cDNA clones which can improve the differential diagnosis
of the small cell tumors. This approach, if successful, will improve
subclassification of other cancer cell types so that the most effective
treatment can be selected. (2)
我们将使用重组DNA技术来选择cDNA克隆,
改善小细胞肿瘤诊断。 这些癌症包括燕麦细胞
癌,许多非霍奇金淋巴瘤,尤文肉瘤,小细胞
神经外胚层起源的肿瘤,胚胎性横纹肌肉瘤,以及
白血病
在许多情况下,肿瘤细胞是原始细胞,其可能难以被识别。
分类,因为没有证据表明成熟的特征。 然而
正确的分类是很重要的,因为治疗往往是不同的
根据恶性肿瘤起源组织的细胞类型,
细胞
现在可以使用重组DNA技术来创造
杂交探针,其可以测定存在于一种细胞类型中的mRNA种类
而不是另一个 理论上,免疫诊断方法也能够
分析任何两种恶性细胞类型可能不同的基因产物,
但只有重组DNA技术才能有效地选择
关键的基因产物,即使它是一种罕见的mRNA物种,只有
两种细胞类型之一
在早期的工作中,我们选择了一组对应于丰富的
在骨髓分化过程中,mRNA受到强烈调控。 这些克隆人将
可用于区分骨髓细胞和淋巴细胞。 我们预计
这组克隆体中的一些成员也可能有助于
将任何造血细胞与其他谱系的细胞区分开。
我们将利用新的杂交-消减重组DNA技术,
选择可改善鉴别诊断的额外cDNA克隆
小细胞肿瘤。 这种方法如果成功,
其他癌细胞类型的亚分类,使最有效的
可以选择治疗。 (二)
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Use of deoxyribonucleic acid probes in the identification of cell origin and detection of cellular contamination in human lymphoblastoid cell lines.
使用脱氧核糖核酸探针鉴定人淋巴母细胞系的细胞起源和检测细胞污染。
- DOI:
- 发表时间:1989
- 期刊:
- 影响因子:0
- 作者:Naeim,F;Jensen,L;Susi,E;Yam,P;Gatti,R;Salser,W;Concannon,P
- 通讯作者:Concannon,P
Informativeness of VNTR genetic markers for detecting chimerism after bone marrow transplantation.
VNTR 遗传标记用于检测骨髓移植后嵌合状态的信息。
- DOI:
- 发表时间:1989
- 期刊:
- 影响因子:0
- 作者:Gatti,RA;Nakamura,Y;Nussmeier,M;Susi,E;Shan,W;Grody,WW
- 通讯作者:Grody,WW
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WINSTON SALSER其他文献
WINSTON SALSER的其他文献
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{{ truncateString('WINSTON SALSER', 18)}}的其他基金
RECOMBINANT DNA DIAGNOSTIC PROBES FOR SMALL CELL CANCERS
小细胞癌重组 DNA 诊断探针
- 批准号:
3181282 - 财政年份:1985
- 资助金额:
$ 11.16万 - 项目类别:
RECOMBINANT DNA DIAGNOSTIC PROBES FOR SMALL CELL CANCERS
小细胞癌重组 DNA 诊断探针
- 批准号:
3181281 - 财政年份:1985
- 资助金额:
$ 11.16万 - 项目类别:
REC-DNA ANALYSIS OF HUMAN HEMATOPOIETIC DIFFERENTIATION
人类造血分化的 REC-DNA 分析
- 批准号:
3170165 - 财政年份:1982
- 资助金额:
$ 11.16万 - 项目类别:
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