AUTOMATED DNA SEQUENCING CORE FACILITY

自动化 DNA 测序核心设施

• 批准号: 3521160
• 负责人: WINSTON SALSER
• 金额: $ 30万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-16 至 1993-07-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521160
• 关键词: DNA; biomedical automation; biomedical equipment purchase; biomedical equipment resource; computer assisted sequence analysis; computer data analysis; computer program /software; nucleic acid sequence; polymerase chain reaction; robotics; spectrometry

The UCLA Jonsson Comprehensive Cancer Center (JCCC) DNA Sequenator Core Facility initiated operations in 1988. Its goal is to provide automated sequencing technology to produce the highest quality sequences in the most cost-effective manner. In a 1987 survey more than 60 UCLA laboratories indicated that they not only used DNA sequencing techniques but felt that the rapid advances in automated DNA sequencing should be developed at UCLA within the Core Facility framework so that, as the technology became appropriate for a greater and greater variety of projects, these techniques would be immediately available to all laboratories with corresponding needs. The number of UCLA laboratories using DNA sequencing techniques or planning to use them in the near future has grown to an estimated 100 at the present time, of which 42 have already used the Core Facility and 89 are participants in this grant because the added capabilities will be important to their research. The creation of this Core Facility has been strongly supported by the Dean of the UCLA School of Medicine and the Dean of Life Sciences, who provided approximately $140,000 in start-up funds in 1988. Additional secure funding, currently $43,418 per year, is available through 1993 from a Cancer Center Core Support Grant. This strong initial support enabled the Core Facility to set records for high accuracy automated sequencing of single strand templates to and beyond 500 nucleotides within three months of receiving the automated sequencing machine in 1988. It must be acknowledged that this is currently a paradoxical field. Even the university laboratory most responsible for developing automated sequencing techniques is currently said to use manual sequencing for most of its research. At UCLA there are many laboratories who could not benefit from the 1st generation automated sequencing equipment but who feel that the 2nd generation equipment requested here overcomes these earlier limitations and will greatly facilitate their work. UCLA's Core Facility has become especially helpful to the great number of laboratories that rely on sequencing but cannot make it the major focus of their research effort. For these laboratories even the first generation equipment drastically cuts costs and gives very high quality sequence. UCLA's Core Facility is widely known for its success in creating an operation which services a large numbers of individual laboratories; for success in areas such as automated sequencing of duplex (supercoiled) templates, which has proved difficult for many; and for achieving a very efficient operation with one of the lowest recharge rates in the world. Several items of the requested equipment are essential to enable the Core Facility to process the expanding volume of samples supplied by the Core Facility users. Also, the superior data analysis possible with the Fast Data Finder will greatly reduce the enormously time-consuming and mind-numbing aspects of data overlap and editing required to produce accurate finished sequences. Without the Fast Data Finder, such analysis is more time consuming than producing the data.

加州大学洛杉矶分校琼森综合癌症中心（JCCC）DNA测序仪 核心设施于1988年开始运营。 它的目标是提供 自动测序技术可产生最高质量的序列 以最具成本效益的方式。 在1987年的一项调查中， 实验室表示，他们不仅使用了DNA测序技术， 但他认为自动DNA测序的快速发展 在加州大学洛杉矶分校的核心设施框架内开发，以便，作为 技术变得适用于越来越多的 项目，这些技术将立即提供给所有 有相应需求的实验室。 加州大学洛杉矶分校的实验室数量 使用DNA测序技术或计划在不久的将来使用它们 目前估计已增加到100个，其中42个已 使用了核心基金，89人参加了这项赠款，因为 增加的能力对他们的研究很重要。 这一核心基金的设立得到了大力支持 加州大学洛杉矶分校医学院院长和生命科学院院长 卫生组织在1988年提供了大约140 000美元的启动资金。 额外 有保障的经费目前每年$43 418，可供1993年以前使用， 癌症中心核心支持补助金 这一有力的初步支持使 核心设施将创下高准确性自动测序的记录 单链模板在三个月内达到或超过500个核苷酸 在1988年收到了自动测序仪。 必须 承认这是目前一个矛盾的领域。 连 大学实验室主要负责开发自动测序 技术目前据说使用手动测序的大部分， research. 在加州大学洛杉矶分校，有许多实验室无法从 第一代自动测序设备，但谁觉得第二代 这里要求的发电设备克服了这些早期的限制， 这将极大地促进他们的工作。 加州大学洛杉矶分校的核心设施已成为 特别是对大量依赖于 测序，但不能使其成为他们研究工作的主要重点。 对于这些实验室，即使是第一代设备也会大幅削减 成本低，并提供非常高质量的序列。 加州大学洛杉矶分校的核心设施广泛 以其成功地创建了一个服务于大型 独立实验室的数量;在自动化等领域取得成功 双链体（超螺旋）模板的测序，这已被证明是困难的 对于许多人来说;并实现一个非常有效的运作与其中之一， 世界上最低的充电率。 所要求的几个项目 设备是必不可少的，使核心设施处理 扩大核心设施用户提供的样本量。 还 快速数据分析仪可实现上级数据分析， 减少数据的巨大耗时和令人麻木的方面 重叠和编辑需要产生准确的成品序列。 如果没有快速数据分析工具， 产生数据。