CORE--DNA SEQUENCING

核心——DNA测序

• 批准号: 6563718
• 负责人: WINSTON SALSER
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563718
• 关键词: DNA; biomedical automation; biomedical facility; computer assisted sequence analysis; computer system hardware; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; robotics

Description: The goal for the DNA Sequencing Shared Resource is to provide high quality DNA sequence analysis at low cost and with rapid turn-around to members of the Cancer Center. The facility operates ABI 373 and 377 automated DNA sequencers, an ABI Catalyst 800 Robotic Work Station, an ABI DNA/RNA synthesizer, and six support computers with appropriate software. Funds are requested to support a portion of salaries of four individuals involved in facility operations and 15 percent of the service contracts for the various apparatus in the facility. The use of the DNA Sequence Facility has increased over 500 percent since the time of the last review. Usage is primarily measured in terms of DNA sequence output, expressed as number of base pairs. The number of investigators that have used the DNA Sequencing Share Resource is a second measure of utilization. Approximately 1.2 million base pairs of sequence were generated in the 1995/1996 fiscal year. Twenty-five Cancer Center members, including 23 with peer-reviewed funded projects, used the facility on 26 funded projects; 52 non-members used it as well. The relative use by members of the Cancer Center, expressed as percentage of bases sequenced, was 44 percent. The total capacity of the shared resource was not provided in the written materials, but at the site visit, it was indicated that the use of the resource had increased further and that it is currently operating at 80 percent of capacity. The applicants anticipate a continued increase in use of the facility. The Howard Hughes Medical Institute at UCLA contributed the ABI 377 automated DNA sequencer to the Shared Resource output.

描述：DNA 测序共享资源的目标是提供 低成本、快速周转的高质量 DNA 序列分析 癌症中心的成员。该设施运行 ABI 373 和 377 自动化 DNA 测序仪、ABI Catalyst 800 机器人工作站、ABI DNA/RNA 合成器，以及六台带有适当软件的支持计算机。资金有 要求支持参与该项目的四名人员的部分工资 设施运营和各种服务合同的 15% 设施内的设备。 自推出以来，DNA 测序设施的使用量增加了 500% 以上 上次审核时间。使用情况主要根据 DNA 序列来衡量 输出，表示为碱基对的数量。调查人员数量 已使用 DNA 测序共享资源是第二个衡量标准 利用率。生成了大约 120 万个碱基对的序列 在1995/1996财政年度。 25 名癌症中心成员，其中 23 名 通过同行评审的资助项目，在 26 个资助项目上使用了该设施； 52 名非会员也使用了它。巨蟹座成员的相对使用 中心（以已测序碱基的百分比表示）为 44%。总计 书面材料中没有提供共享资源的容量，但是 实地考察表明，资源的使用有所增加 此外，目前其产能已达到 80%。这 申请人预计该设施的使用量将持续增加。霍华德酒店 加州大学洛杉矶分校休斯医学院贡献了 ABI 377 自动化 DNA 定序器到共享资源输出。