CORE--DNA SEQUENCING

核心——DNA测序

• 批准号: 6563718
• 负责人: WINSTON SALSER
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563718
• 关键词: DNA; biomedical automation; biomedical facility; computer assisted sequence analysis; computer system hardware; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; robotics

Description: The goal for the DNA Sequencing Shared Resource is to provide high quality DNA sequence analysis at low cost and with rapid turn-around to members of the Cancer Center. The facility operates ABI 373 and 377 automated DNA sequencers, an ABI Catalyst 800 Robotic Work Station, an ABI DNA/RNA synthesizer, and six support computers with appropriate software. Funds are requested to support a portion of salaries of four individuals involved in facility operations and 15 percent of the service contracts for the various apparatus in the facility. The use of the DNA Sequence Facility has increased over 500 percent since the time of the last review. Usage is primarily measured in terms of DNA sequence output, expressed as number of base pairs. The number of investigators that have used the DNA Sequencing Share Resource is a second measure of utilization. Approximately 1.2 million base pairs of sequence were generated in the 1995/1996 fiscal year. Twenty-five Cancer Center members, including 23 with peer-reviewed funded projects, used the facility on 26 funded projects; 52 non-members used it as well. The relative use by members of the Cancer Center, expressed as percentage of bases sequenced, was 44 percent. The total capacity of the shared resource was not provided in the written materials, but at the site visit, it was indicated that the use of the resource had increased further and that it is currently operating at 80 percent of capacity. The applicants anticipate a continued increase in use of the facility. The Howard Hughes Medical Institute at UCLA contributed the ABI 377 automated DNA sequencer to the Shared Resource output.

描述：DNA测序共享资源的目标是提供 以低成本和快速周转进行高质量DNA序列分析， 癌症中心的成员。该设施操作ABI 373和377自动化 DNA测序仪，ABI Catalyst 800机器人工作站，ABI DNA/RNA 合成器和六台配有适当软件的辅助计算机。资金 要求支付四个人的部分薪金， 设施运营和15%的服务合同， 设施中的设备。 自2000年以来，DNA测序设施的使用增加了500%以上。 上次审查的时间。使用率主要以DNA序列来衡量 输出，表示为碱基对的数量。调查人员的数量， 使用DNA测序共享资源是第二个衡量 利用率产生了大约120万个碱基对的序列 1995/1996财政年度。25名癌症中心成员，包括23名 对于同行审查的供资项目，在26个供资项目上使用了该机制; 52个非成员也使用了它。巨蟹座成员的相对使用 中心，表示为测序的碱基的百分比，是44%。总 书面材料中没有提供共享资源的能力，但 在实地访问时，有人指出，资源的使用有所增加 目前，它正在以80%的能力运作。的 申请人预期使用该设施的人数会继续增加。那个霍华德 加州大学洛杉矶分校的休斯医学研究所贡献了ABI 377自动DNA 序列器到共享资源输出。