CORE--DNA SEQUENCING

核心——DNA测序

• 批准号: 6563718
• 负责人: WINSTON SALSER
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563718
• 关键词: DNA; biomedical automation; biomedical facility; computer assisted sequence analysis; computer system hardware; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; robotics

Description: The goal for the DNA Sequencing Shared Resource is to provide high quality DNA sequence analysis at low cost and with rapid turn-around to members of the Cancer Center. The facility operates ABI 373 and 377 automated DNA sequencers, an ABI Catalyst 800 Robotic Work Station, an ABI DNA/RNA synthesizer, and six support computers with appropriate software. Funds are requested to support a portion of salaries of four individuals involved in facility operations and 15 percent of the service contracts for the various apparatus in the facility. The use of the DNA Sequence Facility has increased over 500 percent since the time of the last review. Usage is primarily measured in terms of DNA sequence output, expressed as number of base pairs. The number of investigators that have used the DNA Sequencing Share Resource is a second measure of utilization. Approximately 1.2 million base pairs of sequence were generated in the 1995/1996 fiscal year. Twenty-five Cancer Center members, including 23 with peer-reviewed funded projects, used the facility on 26 funded projects; 52 non-members used it as well. The relative use by members of the Cancer Center, expressed as percentage of bases sequenced, was 44 percent. The total capacity of the shared resource was not provided in the written materials, but at the site visit, it was indicated that the use of the resource had increased further and that it is currently operating at 80 percent of capacity. The applicants anticipate a continued increase in use of the facility. The Howard Hughes Medical Institute at UCLA contributed the ABI 377 automated DNA sequencer to the Shared Resource output.

描述：DNA测序共享资源的目标是提供 高质量的DNA序列分析，成本低，运行速度快 癌症中心的成员。该设施运行ABI 373和377自动化 DNA测序仪，ABI Catalyst 800机器人工作站，ABI DNA/RNA 合成器，以及六台装有适当软件的支持计算机。基金是 被要求支持四名参与 设施运营和各种服务合同的15% 设施中的设备。 DNA测序设备的使用自 上次审阅的时间。使用率主要是根据DNA序列来衡量的 输出，以碱基对的数量表示。调查人员的数量 已使用DNA测序共享资源是衡量 利用率。产生了大约120万个碱基对的序列 在1995/1996财政年度。25名癌症中心成员，其中包括23名 对于同行审查的资助项目，在26个资助项目上使用了该融资机制； 52个非会员也使用了它。巨蟹座成员的相对使用 中心，以测序的碱基百分比表示，为44%。总数 书面材料中没有提供共享资源的能力，但 在现场访问中，有人指出资源的使用有所增加。 此外，它目前正以80%的运力运行。这个 申请者预计该设施的使用量将继续增加。霍华德 加州大学洛杉矶分校的休斯医学院贡献了ABI 377自动DNA 共享资源输出的定序器。