13TSB_TIBio: Enabling Technologies for Advanced Multiphoton Microscopy

13TSB_TIBio：先进多光子显微镜的实现技术

• 批准号: BB/M003841/1
• 负责人: Julian Moger
• 金额: $ 18.34万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM003841%2F1
• 关键词: 13TSB_TIBio; Enabling; Technologies; Advanced; Multiphoton

A major challenge in fundamental biological research and the development of biotechnology is to measure heterogeneity in chemical composition at the cellular and subcellular scale in living cells and tissues. Gaining the ability to visualise the distribution of compounds at this scale would represent a major advancement. The current standard in biological imaging, fluorescence microscopy, provides chemically specific image contrast by molecularly targeted probes. However, these probes are too bulky for labelling small molecules such as lipids, carbohydrates, metabolites and many drugs that play essential roles in the function of living cells and tissues. Coherent Raman scattering (CRS) microscopy has emerged as powerful tool to generate signals from vibrational spectroscopy to provide label-free mapping of biomolecules in real-time. However, due to the specialised laser requirements, this technique is currently confined to a handful of specialised laboratories with the resources and expertise to operate such laser systems. This project aims to develop a simple, compact module that will upgrade pulsed lasers currently used in biological microscopes to provide wider access to CRS imaging.

基础生物学研究和生物技术发展中的一个主要挑战是在活细胞和组织的细胞和亚细胞尺度上测量化学组成的异质性。获得在这种规模下可视化化合物分布的能力将是一个重大进步。目前生物成像的标准，荧光显微镜，通过分子靶向探针提供化学特异性图像对比。然而，这些探针对于标记在活细胞和组织的功能中起重要作用的小分子如脂质、碳水化合物、代谢物和许多药物来说太笨重。相干拉曼散射（CRS）显微镜已经成为一种强大的工具，从振动光谱产生信号，以提供实时的生物分子的无标记映射。然而，由于专业的激光要求，这项技术目前仅限于少数几个拥有操作这种激光系统的资源和专业知识的专业实验室。该项目旨在开发一种简单紧凑的模块，将升级目前用于生物显微镜的脉冲激光器，以提供更广泛的CRS成像。