Frequency-Modulated Stimulated Raman Scattering Microscopy for Label-Free Functional Imaging In-Planta

用于植物内无标记功能成像的调频受激拉曼散射显微镜

• 批准号: BB/K013602/1
• 负责人: Julian Moger
• 金额: $ 15.22万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK013602%2F1
• 关键词: Frequency; Modulated; Stimulated; Raman; Scattering

The ability to create images of living cells deep within biological tissues and to identify chemical species within them has been a dream for scientists in many disciplines for many years. This dream is becoming reality - physicists are developing cutting-edge optical microscopy techniques that allow biomedical scientists to look inside living tissues and identify biomolecules based on subtle differences in the way they interact with light. Unfortunately, the plant science community has not been able to benefit from these techniques. Plants have evolved to be highly efficient absorbers of light, and contain pigments that generate an optical signal that overwhelms the subtle interactions between the light and the biomolecules of interest. We proposed to develop a new detection scheme that will overcome the fundamental limitations of imaging in the presence of strong optical absorption and allow both academic and industrial researchers to benefit from this cutting-edge technology.

多年来，能够在生物组织深处创造活细胞的图像，并识别其中的化学物质，一直是许多学科的科学家的梦想。这个梦想正在成为现实——物理学家正在开发尖端的光学显微镜技术，使生物医学科学家能够观察活体组织内部，并根据生物分子与光相互作用方式的细微差异来识别生物分子。不幸的是，植物科学界未能从这些技术中受益。植物已经进化成为高效的光吸收者，并且含有能够产生光信号的色素，这种光信号压倒了光和感兴趣的生物分子之间微妙的相互作用。我们建议开发一种新的检测方案，以克服在强光吸收存在下成像的基本限制，并使学术和工业研究人员都能从这项尖端技术中受益。

项目成果

Making microscopy count: quantitative light microscopy of dynamic processes in living plants.

让显微镜发挥作用：活体植物动态过程的定量光学显微镜。

• DOI: 10.1111/jmi.12403
• 发表时间: 2016
• 期刊: Journal of microscopy
• 影响因子: 2
• 作者: Fricker MD

Making open data work for plant scientists.

• DOI: 10.1093/jxb/ert273
• 发表时间: 2013-11
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Leonelli S;Smirnoff N;Moore J;Cook C;Bastow R
• 通讯作者: Bastow R

An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.

• DOI: 10.3389/fpls.2014.00140
• 发表时间: 2014
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Littlejohn GR;Mansfield JC;Christmas JT;Witterick E;Fricker MD;Grant MR;Smirnoff N;Everson RM;Moger J;Love J
• 通讯作者: Love J

Imaging plant tissues with coherent Raman scattering microscopy

使用相干拉曼散射显微镜对植物组织进行成像

• DOI: 10.1117/12.2001696
• 发表时间: 2013
• 作者: Moger J