Frequency-Modulated Stimulated Raman Scattering Microscopy for Label-Free Functional Imaging In-Planta

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The ability to create images of living cells deep within biological tissues and to identify chemical species within them has been a dream for scientists in many disciplines for many years. This dream is becoming reality - physicists are developing cutting-edge optical microscopy techniques that allow biomedical scientists to look inside living tissues and identify biomolecules based on subtle differences in the way they interact with light. Unfortunately, the plant science community has not been able to benefit from these techniques. Plants have evolved to be highly efficient absorbers of light, and contain pigments that generate an optical signal that overwhelms the subtle interactions between the light and the biomolecules of interest. We proposed to develop a new detection scheme that will overcome the fundamental limitations of imaging in the presence of strong optical absorption and allow both academic and industrial researchers to benefit from this cutting-edge technology.