13 ERA IB: Integrated Process and Cell Refactoring Systems (IPCRES) for Enhanced Industrial Biotechnology.

低价值废弃甘油的增值可以改变生物柴油生产的经济性。通过生物催化实现这一目标的关键挑战是甘油转化为产品的速度，整合生物过程步骤以及宿主菌株对粗原料污染物的耐受性。我们是工业和学术生物过程，细胞工程，组学和生物系统专家。我们将在酿酒酵母和毕氏酵母中引入途径，从甘油中生产两种有价值的化合物：手性氨基醇（CAA）和1,2-丙二醇（PDO）。细胞将以高通量微尺度工艺技术和规模为特征。转录组学和代谢组学数据将与过程见解相结合，以指示途径和宿主“底盘”的重构。这些步骤将在第二次迭代单元中重复，以获得更好的性能。新的菌株、工艺和综合方法将用于生物柴油和工业生物技术部门。

Valorisation of low-value waste glycerol could transform the economics of biodiesel production.Key challenges for achieving this with biocatalysis are the rate of glycerol conversion to products, integrating bioprocess steps and tolerance of crude feedstock contaminants by host strains.We are industrial and academic bioprocess, cell-engineering, omics and bio-systems specialists. We will port pathways into Saccharomyces cerevisiae and Pichia pastoris for production of two valuable compounds from glycerol: chiral amino-alcohols (CAA) and 1,2-propanediol (PDO).Cells will be characterised with high throughput micro-scale process techniques and at scale. Transcriptomic and metabolomic data will be combined with process insights to dictate refactoring of both pathways and host 'chassis'. These steps will be repeated for second-iteration cells, driving better performance. New strains, processes and integrated methods will be of use to biodiesel and Industrial Biotechnology sectors.