ERA IB 5 A Synthetic Biology approach for bacterial bioconversion of lignin into renewable chemicals (LIGBIO)

ERA IB 5 将木质素细菌生物转化为可再生化学品的合成生物学方法 (LIGBIO)

• 批准号: BB/M025772/1
• 负责人: Timothy Bugg
• 金额: $ 45.48万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM025772%2F1
• 关键词: ERA; IB; Synthetic; Biology; approach

Lignin is a polymer found as 15-30% of plant biomass, and is produced industrially as a low-value by-product of pulp/paper manufacture and biofuel production. It contains phenolic monomer units, hence it represents a possible renewable source of aromatic chemicals that are needed industrially to make plastics, pharmaceuticals and agrochemicals, currently derived from crude oil. As part of the effort to develop a sustainable European bio-based chemical industry, we wish to convert lignin into renewable chemicals, however, due to its highly inert structure, no commercial process has yet been developed for conversion of lignin to renewable chemicals. Recently Prof. Bugg's group at Warwick have discovered two novel bacterial lignin-degrading enzymes, and have shown that the lignin breakdown pathways in Rhodococcus jostii RHA1 can be modified genetically in order to generate vanillin, a chemical used in the food/flavour industry. However, at present the metabolic pathways and regulatory systems for lignin breakdown in bacteria are not well understood. Therefore, in this project we will study Pseudomonas putida KT2440, a lignin-degrading bacterium whose genome has been sequenced and whose aromatic metabolic network is the best known in bacteria, to elucidate the metabolic and regulatory networks for lignin degradation. We will then use this knowledge to reprogramme this bacterium, using high activity lignin-degrading enzymes, to produce two target chemicals that are of interest for the production of renewable bioplastics. With industrial partner Biome Bioplastics, we will then develop a fermentation-based process for the production of renewable aromatic chemicals from lignin.

木质素是一种聚合物，占植物生物量的15-30％，并且在工业上以纸浆/纸制造和生物燃料生产的低价值副产品生产。它包含酚类单体单元，因此代表了可能在工业上生产塑料，药物和农业化学物质所需的芳香化学物质的可能来源，目前是从原油中得出的。为了开发可持续的欧洲生物化学工业的努力，我们希望将木质素转化为可再生化学物质，但是由于其高度惰性的结构，尚未开发出用于将木质素转化为可再生化学物质的商业过程。最近，布格教授在沃里克（Warwick）的小组发现了两种新型的细菌木质素降解酶，并表明，若迪克斯（Rhodococcus jostii Rha1）中的木质素分解途径可以在遗传上修饰，以产生香草蛋白，这是一种用于食品/风味行业中的化学物质。但是，目前尚不清楚用于细菌木质素分解的代谢途径和调节系统。因此，在这个项目中，我们将研究一种木质素降解细菌Putida Kt2440，其基因组已被测序，其芳族代谢网络是细菌中最著名的，以阐明木质素降解的代谢和调节网络。然后，我们将使用这些知识来使用高活性木质素降解酶对这种细菌进行重编程，以产生两种目标化学物质，这些化学物质对生产可再生生物塑料的生产感兴趣。借助工业伙伴生物群落生物塑料，我们将开发一种基于发酵的过程，用于从木质素中生产可再生芳香化学物质。

项目成果

Resource Recovery from Wastes - Towards a Circular Economy

废物资源回收 - 迈向循环经济

• DOI: 10.1039/9781788016353-00113
• 发表时间: 2019
• 作者: Lag-Brotons A

Identification of an extracellular bacterial flavoenzyme that can prevent re-polymerisation of lignin fragments.

• DOI: 10.1016/j.bbrc.2016.10.144
• 发表时间: 2017
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Rahman Rahmanpour;Lloyd King;T. Bugg

Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates.

荧光假单胞菌过氧化物酶 Dyp1B 的蛋白质工程用于氧化酚类和聚合木质素底物。

• DOI: 10.1016/j.enzmictec.2019.01.002
• 发表时间: 2019
• 期刊: Enzyme and microbial technology
• 影响因子: 3.4
• 作者: Rahman Pour R

Characterisation of Dyp-type peroxidases from Pseudomonas fluorescens Pf-5: Oxidation of Mn(II) and polymeric lignin by Dyp1B

• DOI: 10.1016/j.abb.2014.12.022
• 发表时间: 2015-05-15
• 期刊: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
• 影响因子: 3.9
• 作者: Rahmanpour, Rahman;Bugg, Timothy D. H.
• 通讯作者: Bugg, Timothy D. H.

Sphingobacterium sp. T2 Manganese Superoxide Dismutase Catalyzes the Oxidative Demethylation of Polymeric Lignin via Generation of Hydroxyl Radical.

• DOI: 10.1021/acschembio.8b00557
• 发表时间: 2018-09
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: G. M. Rashid;Xiaoyang Zhang;R. Wilkinson;V. Fülöp;B. Cottyn;S. Baumberger;T. Bugg