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DIFFERENTIATION ANTIGENS OF HUMAN PANCREATIC TUMORS

人胰腺肿瘤的分化抗原

基本信息

批准号：
3191180
负责人：
RICHARD S METZGAR
金额：
$ 16.91万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-06-01 至 1992-05-31
项目状态：
已结题

项目摘要

The objectives of this research are to better define the antigens and genes of human pancreatic adenocarcinoma cells. Of particular interest are those genes and gene products related to transformation and differentiation of pancreatic ductal epithelial cells. To achieve the goals we propose to produce polyclonal and monoclonal antibodies to antigens of human pancreatic tumor lines which are related to differentiation. Some of these antigens are already defined by our existing murine DU-PAN monoclonal antibodies while others will be new. These studies also require molecular characterization and purification of the antigens. In addition, we propose to establish and characterize a unique series of tissue culture cell lines and clones which demonstrate differential expression of some genes and gene products related to differentiation and transformation of pancreatic ductal cells. These approaches include further characterization of existing clones of a pancreatic tumor cell line and production and characterization of new clones including clones with high metastatic potential. We will also attempt induction of differentiation with chemical agents and will produce and characterize human-hamster hybrid lines and clones. Finally, we propose to establish some cDNA and expression libraries which can be utilized for cloning genes coding for antigens defined by our antibodies and for identification of unique genes related to differentiation, metastases or transformation expressed in the clones and lines generated from this study.

这项研究的目的是更好地定义抗原和人胰腺癌细胞的基因。的特别感兴趣的是与这些基因和基因产物相关的基因和基因产物胰腺导管上皮细胞的转化与分化细胞。为了实现我们计划生产的多克隆和抗人胰腺肿瘤抗原的单抗与分化相关的品系。其中一些抗原已经被我们现有的小鼠DU-PAN单抗所定义抗体，而其他的将是新的。这些研究还需要抗原的分子表征和纯化。在……里面此外，我们建议建立和表征一个独特的系列组织培养细胞系和克隆一些相关基因和基因产物的差异表达胰腺导管细胞的分化转化。这些方法包括对现有的胰腺肿瘤细胞系的克隆及其生产和鉴定包括高密度克隆在内的新克隆的特性研究转移潜能。我们还将尝试诱导与化学试剂的差异化，并将产生和描述人-仓鼠杂交品系和克隆的特征。最后，我们建议建立一些基因文库和表达文库可用于克隆编码由我们的抗体和鉴定与之相关的独特基因分化、转移或转化表达于从这项研究中产生的克隆和系。