IMMUNOLOGICAL STUDIES OF MEMBRANE ANTIGENS

膜抗原的免疫学研究

• 批准号: 3179478
• 负责人: RICHARD S METZGAR
• 金额: $ 15.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1975
• 资助国家: 美国
• 起止时间: 1975-05-01 至 1990-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3179478
• 关键词: B lymphocyte; T lymphocyte; chromatography; electrofocusing; evolution; human tissue; immunochemistry; interleukin 2; leukopoietic factor; lymphokines; macrophage; membrane structure; monocyte; myelocyte; radiotracer; serology /serodiagnosis; surface antigens; tissue /cell culture

The long-term objective of these studies is to define selected antigens of human cells using antibodies produced in non-human primates. The immunological perspective of the non-human primates should allow the recognition of epitopes on these antigens which may be difficult to be detected by lower mammalian species. The specific aims for this renewal period are to: (1) establish the optimal conditions and method for production of chimpanzee monoclonal antibodies to selected human normal and malignant cell antigens; (2) establish the optimal conditions and methods for the in vitro transfection or infection of chimpanzee cells with oncogenes or human tumor DNA; and (3) from aim 2, establish the immunogenicity of tumorigenicity of chimpanzee cells that show evidence of gene insertion or changes in vitro growth characteristics. Specifically, aim 1 will evaluate various methods of chimpanzee B-cell fusion such as electrofusion and PEG, varying the conditions for fusion and growth as well as the parental human and murine myeloma lines to be used as fusion partners. In particular, aim 2 will evaluate various in vitro methods of gene insertion including transfection and infection of chimpanzee fibroblast, epithelial cells, or lymphocytes. Aim 3 will utilize standard injection and serological procedures adapted in this laboratory to induce and measure the autologous immune response in chimpanzees to human gene insertion, their in vitro growth properties, and the nature of the insertion technique. (CS)

这些研究的长期目标是确定选定的抗原 人类细胞使用非人类灵长类动物产生的抗体。 这 非人类灵长类动物的免疫学观点应该允许 识别这些抗原上可能难以识别的表位 由低等哺乳动物物种检测到。 本次更新的具体目标 期间的目标是： (1) 建立最佳的条件和方法 生产黑猩猩单克隆抗体以选择人类正常和 恶性细胞抗原； (2)建立最优条件和方法 用于体外转染或感染黑猩猩细胞 癌基因或人类肿瘤DNA； (3) 根据目标 2，建立 黑猩猩细胞致瘤性的免疫原性表明 基因插入或改变体外生长特性。 具体来说，目标 1 将评估黑猩猩 B 细胞的各种方法 融合，例如电融合和 PEG，改变融合条件和 生长以及亲本人类和小鼠骨髓瘤系用作 融合伙伴。 特别是，目标 2 将评估各种体外 基因插入方法包括转染和感染 黑猩猩成纤维细胞、上皮细胞或淋巴细胞。 目标3将 使用本标准中采用的标准注射和血清学程序 实验室诱导和测量自体免疫反应 黑猩猩对人类基因的插入，它们的体外生长特性，以及 插入技术的性质。 （CS）