DIFFERENTIATION ANTIGENS OF HUMAN PANCREATIC TUMORS

人胰腺肿瘤的分化抗原

• 批准号: 3191178
• 负责人: RICHARD S METZGAR
• 金额: $ 16.75万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3191178
• 关键词: adenocarcinoma; cell differentiation; chemical carcinogen; clone cells; epithelium; gene expression; genetic library; heterokaryon; human tissue; laboratory mouse; laboratory rabbit; metastasis; monoclonal antibody; neoplasm /cancer genetics; neoplastic cell; nucleic acid probes; pancreas neoplasms; pancreatic duct; tumor antigens

The objectives of this research are to better define the antigens and genes of human pancreatic adenocarcinoma cells. Of particular interest are those genes and gene products related to transformation and differentiation of pancreatic ductal epithelial cells. To achieve the goals we propose to produce polyclonal and monoclonal antibodies to antigens of human pancreatic tumor lines which are related to differentiation. Some of these antigens are already defined by our existing murine DU-PAN monoclonal antibodies while others will be new. These studies also require molecular characterization and purification of the antigens. In addition, we propose to establish and characterize a unique series of tissue culture cell lines and clones which demonstrate differential expression of some genes and gene products related to differentiation and transformation of pancreatic ductal cells. These approaches include further characterization of existing clones of a pancreatic tumor cell line and production and characterization of new clones including clones with high metastatic potential. We will also attempt induction of differentiation with chemical agents and will produce and characterize human-hamster hybrid lines and clones. Finally, we propose to establish some cDNA and expression libraries which can be utilized for cloning genes coding for antigens defined by our antibodies and for identification of unique genes related to differentiation, metastases or transformation expressed in the clones and lines generated from this study.

本研究的目的是更好地确定抗原 和人胰腺癌细胞的基因。 的 特别令人感兴趣的是那些基因和基因产物， 胰腺导管上皮细胞的转化和分化 细胞 为了实现这一目标，我们建议生产多克隆和 抗人胰腺肿瘤抗原单克隆抗体 与分化有关的线。 这些抗原中的一些 已经被我们现有的鼠DU-PAN单克隆抗体所定义 抗体，而另一些将是新的。 这些研究还要求 抗原的分子表征和纯化。 在 此外，我们建议建立和表征一个独特的系列， 组织培养细胞系和克隆， 一些基因和基因产物的差异表达 胰腺导管细胞的分化和转化。 这些方法包括进一步表征现有的 胰腺肿瘤细胞系的克隆和生产， 新克隆的表征，包括具有高 转移潜能 我们还将尝试归纳 与化学试剂的差异，并将产生和 表征人-仓鼠杂交系和克隆。 最后我们 建议建立一些cDNA和表达文库， 可用于克隆编码抗原的基因，所述抗原定义如下 我们的抗体，并用于识别与 表达的分化、转移或转化。 本研究产生的克隆和品系。