High performance mass spectrometry at the University of Birmingham

伯明翰大学的高性能质谱仪

• 批准号: BB/M012492/1
• 负责人: Helen Cooper
• 金额: $ 54.18万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM012492%2F1
• 关键词: performance; mass; spectrometry; University; Birmingham

Mass spectrometry is well-established as a technique for characterisation of molecular structure. The chief reason for the success of mass spectrometry is that every molecule has a mass, unlike other properties sometimes measured in bioanalytical chemistry, such as a magnetic moment or absorbance of light at a particular wavelength. The mass of a molecule depends on the composition of its atoms, as each atom has a unique mass. As long as the accuracy of the mass spectrometer is high enough, then measurement of a molecule's mass allows determination of the elemental composition. The more complex the sample, either in terms of the number of components or in terms of the size of the molecule (the number of atoms), the more challenging is mass analysis. This fact is due to overlapping signals from different molecules or versions of the same molecule (isotopomers) and in order to address the problem, a very high resolution mass spectrometer is required.Biological research is overflowing with sample types that require high resolution mass spectrometry: Metabolomic samples contain thousands of low molecular weight species. Proteins are large molecules associated with a vast array of chemical modifications, and which form loosely-bound complexes with themselves, other proteins and other molecule types. The aim of this proposal is to make available the latest developments in the highest performance type of mass spectrometry - Fourier transform ion cyclotron resonance mass spectrometry - to biological research at the University of Birmingham and beyond. The proposal brings together a broad range of researchers from diverse disciplines to address several key strategic priorities for BBSRC (Healthy ageing across the life course, Synthetic biology, Combatting antimicrobial resistance, Food, nutrition & health, Technology for the biosciences).

质谱法作为表征分子结构的技术是公认的。质谱法成功的主要原因是每个分子都有质量，不像生物分析化学中有时测量的其他性质，例如磁矩或特定波长的光吸收。分子的质量取决于其原子的组成，因为每个原子都有唯一的质量。只要质谱仪的精度足够高，那么测量分子的质量就可以确定元素组成。样品越复杂，无论是组分数量还是分子大小（原子数），质量分析的挑战性就越大。这一事实是由于来自不同分子或同一分子（同位素异构体）版本的重叠信号造成的，为了解决这个问题，需要非常高分辨率的质谱仪。生物研究中充满了需要高分辨率质谱的样本类型：代谢组学样本包含数千种低分子量物质。蛋白质是与大量化学修饰相关的大分子，并且与自身、其他蛋白质和其他分子类型形成松散结合的复合物。该提案的目的是将最高性能类型的质谱-傅立叶变换离子回旋共振质谱-的最新发展用于伯明翰大学及其他地区的生物研究。该提案汇集了来自不同学科的广泛研究人员，以解决BBSRC的几个关键战略优先事项（整个生命过程中的健康老龄化，合成生物学，对抗抗菌素耐药性，食品，营养与健康，生物科学技术）。

项目成果

Structural Analysis of 14-3-3-ζ-Derived Phosphopeptides Using Electron Capture Dissociation Mass Spectrometry, Traveling Wave Ion Mobility Spectrometry, and Molecular Modeling.

使用电子捕获解离质谱、行波离子淌度光谱和分子建模对 14-3-3-γ-衍生磷酸肽进行结构分析。

• DOI: 10.1021/acs.jpcb.9b08506
• 发表时间: 2020
• 期刊: The journal of physical chemistry. B
• 作者: Simmonds,AnnaL;Lopez-Clavijo,AndreaF;Winn,PeterJ;Russell,DavidH;Styles,IainB;Cooper,HelenJ
• 通讯作者: Cooper,HelenJ

Electron capture dissociation mass spectrometry of phosphopeptides: Arginine and phosphoserine

• DOI: 10.1016/j.ijms.2015.07.024
• 发表时间: 2015-11-15
• 期刊: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
• 影响因子: 1.8
• 作者: Lopez-Clavijo, Andrea F.;Duque-Daza, Carlos A.;Cooper, Helen J.
• 通讯作者: Cooper, Helen J.

Workflow for fast lipid tissue screening using LESA-FT-ICR-MS

• DOI: 10.1039/c8ay02739k
• 发表时间: 2019-05-14
• 期刊: ANALYTICAL METHODS
• 影响因子: 3.1
• 作者: Haler, Jean R. N.;Sisley, Emma K.;Fernandez-Lima, Francisco
• 通讯作者: Fernandez-Lima, Francisco