A new mass spectrometer for structural proteomics and protein imaging

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Proteins are large molecules associated with a vast array of chemical modifications, and which form loosely-bound complexes with themselves, other proteins and other molecules. Proteins work together as machines to perform all the processes required for life. Their functions are intrinsically linked to their overall structure. Mass spectrometry is an analytical technique which enables us to track proteins to understand more about how they work. The global analysis of protein structure is known as structural proteomics, and makes use of mass spectrometry.The aim of this proposal is to install the most advanced mass spectrometer for structural proteomics at the University of Birmingham. The instrument offers high sensitivity, high mass accuracy, and high mass resolving power. The mass spectrometer will also be equipped with the latest ion mobility spectrometry technology, the FAIMS Pro. This device improves the sensitivity and separation capabilities, meaning that it is possible to delve deeper and wider into the proteome. Importantly, the instrument enables cross-linking experiments, providing new capabilities for mapping the architecture of protein complexes. Additionally, the instrument will bring new opportunities for improved mapping of intact protein distribution (mass spectrometry imaging) in tissue and in living microbial colonies.The proposal brings together a broad range of researchers from the University of Birmingham and the wider Midlands (University of Nottingham, University of Leicester) from diverse disciplines to address several key strategic priorities for BBSRC (Healthy ageing across the life course, Combatting antimicrobial resistance, Sustainably enhancing agricultural production, Technology for the biosciences, and Data driven biology).