A new mass spectrometer for structural proteomics and protein imaging

用于结构蛋白质组学和蛋白质成像的新型质谱仪

• 批准号: BB/S019456/1
• 负责人: Helen Cooper
• 金额: $ 79.17万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS019456%2F1
• 关键词: new; mass; spectrometer; structural; proteomics

Proteins are large molecules associated with a vast array of chemical modifications, and which form loosely-bound complexes with themselves, other proteins and other molecules. Proteins work together as machines to perform all the processes required for life. Their functions are intrinsically linked to their overall structure. Mass spectrometry is an analytical technique which enables us to track proteins to understand more about how they work. The global analysis of protein structure is known as structural proteomics, and makes use of mass spectrometry.The aim of this proposal is to install the most advanced mass spectrometer for structural proteomics at the University of Birmingham. The instrument offers high sensitivity, high mass accuracy, and high mass resolving power. The mass spectrometer will also be equipped with the latest ion mobility spectrometry technology, the FAIMS Pro. This device improves the sensitivity and separation capabilities, meaning that it is possible to delve deeper and wider into the proteome. Importantly, the instrument enables cross-linking experiments, providing new capabilities for mapping the architecture of protein complexes. Additionally, the instrument will bring new opportunities for improved mapping of intact protein distribution (mass spectrometry imaging) in tissue and in living microbial colonies.The proposal brings together a broad range of researchers from the University of Birmingham and the wider Midlands (University of Nottingham, University of Leicester) from diverse disciplines to address several key strategic priorities for BBSRC (Healthy ageing across the life course, Combatting antimicrobial resistance, Sustainably enhancing agricultural production, Technology for the biosciences, and Data driven biology).

蛋白质是与大量化学修饰相关的大分子，并且与自身、其他蛋白质和其他分子形成松散结合的复合物。蛋白质像机器一样协同工作，完成生命所需的所有过程。它们的职能与其总体结构有着内在的联系。质谱是一种分析技术，使我们能够跟踪蛋白质，以了解更多关于它们如何工作。对蛋白质结构的全面分析被称为结构蛋白质组学，并利用质谱法。本提案的目的是在伯明翰大学安装最先进的结构蛋白质组学质谱仪。该仪器具有高灵敏度、高质量准确度和高质量分辨能力。质谱仪还将配备最新的离子迁移谱技术FAIMS Pro。该设备提高了灵敏度和分离能力，这意味着可以更深入和更广泛地研究蛋白质组。重要的是，该仪器能够进行交联实验，为绘制蛋白质复合物的结构提供了新的能力。此外，该仪器将为改进完整蛋白质分布的绘图带来新的机会（质谱成像）在组织和活的微生物菌落中。该提案汇集了来自伯明翰大学和更广泛的中部地区的广泛研究人员（诺丁汉大学，莱斯特大学）从不同的学科，以解决BBSRC的几个关键战略重点（整个生命过程中的健康老龄化，对抗抗生素耐药性，可持续地提高农业生产，生物科学技术和数据驱动的生物学）。

项目成果

High-throughput deconvolution of native protein mass spectrometry imaging datasets for mass domain analysis.

用于质量域分析的天然蛋白质质谱成像数据集的高通量反卷积。

• DOI: 10.26434/chemrxiv-2023-1zg89-v2
• 发表时间: 2023
• 作者: Hale O

Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue.

• DOI: 10.1021/acs.analchem.1c05353
• 发表时间: 2022-04-12
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Hale, Oliver J.;Hughes, James W.;Sisley, Emma K.;Cooper, Helen J.
• 通讯作者: Cooper, Helen J.

Probing heavy metal binding to phycobiliproteins.

探测重金属结合与植物脂蛋白的结合。

• DOI: 10.1111/febs.16396
• 发表时间: 2022-08
• 期刊: The FEBS journal

Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue

对直接来自晶状体组织的完整膜蛋白组装体和可溶性蛋白组装体进行天然环境质谱分析

• DOI: 10.1002/ange.202201458
• 发表时间: 2022
• 期刊: Angewandte Chemie
• 作者: Hale O

FAIMS Enhances the Detection of PTM Crosstalk Sites.

• DOI: 10.1021/acs.jproteome.1c00721
• 发表时间: 2022-04-01
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Adoni KR;Cunningham DL;Heath JK;Leney AC
• 通讯作者: Leney AC