EFFECTS OF MORPHINE ON IDENTIFIED CNS PEPTIDERGIC NEURON

吗啡对脑内几种神经内分泌功能的影响 将在本提案中进行研究。 五种神经肽系统已经被 选择;这些是（1）β-内啡肽（POMC）神经元（对大脑至关重要）， 吗啡的中枢作用），（2）α-MSH特异性系统，（3） 加压素（AVP），（4）促肾上腺皮质激素释放因子（CRF），和（5） 促甲状腺激素释放因子（TRF）。 一种新开发的隔离技术 将用于制备POMC、AVP和CRF的纯群体的培养物 来自正常和吗啡处理的动物的下丘脑的神经元。 将详细研究β-内啡肽（POMC）神经元： (1)早期翻译后加工，以及POMC乙酰化后 肽，特别是β-内啡肽和α-MSH，（2）与 TRF可能是内源性拮抗剂，（3）胃，可能共存 POMC神经元中的阿片受体;（4）它们的（阿片）突触前和突触后受体。

The effects of morphine upon several neuroendocrine functions of the brain will be studied in this proposal. Five neuropeptide systems have been chosen; these are (1) the beta-endorphin (POMC) neurons (crucial to the central effects of morphine), (2) the alpha MSH-specific system, (3) vasopressin (AVP, (4) corticotropin releasing factor (CRF), and (5) thyrotropin releasing factor (TRF). A newly developed isolation technique will be used to prepare cultures of pure populations of POMC, AVP, and CRF neurons from the hypothalami of normal and morphine-treated animals. Beta-endorphin (POMC) neurons will be studied in detail with respect to: (1) early post-translational processing, and upon acetylation of POMC peptides, particularly beta-endorphin and alpha-MSH, (2) interactions with TRF which may be an endogenous antagonist, (3) gastric, which may coexist in the POMC neuron, and (4) their (opiate) pre- and postsynaptic receptors.