EFFECTS OF MORPHINE ON IDENTIFIED CNS PEPTIDERGIC NEURON

吗啡对已确定的中枢神经系统肽能神经元的影响

• 批准号: 3209238
• 负责人: KARL M KNIGGE
• 金额: $ 9.06万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3209238
• 关键词: acetylation; brain metabolism; corticotropin releasing factor; gastrins; genetic translation; high performance liquid chromatography; hypothalamus; immunochemistry; melanocyte stimulating hormone; morphine; narcotics; neuroendocrine system; neuropharmacology; radioimmunoassay; secretion; thyrotropin releasing hormone; tissue /cell culture; vasopressins

The effects of morphine upon several neuroendocrine functions of the brain will be studied in this proposal. Five neuropeptide systems have been chosen; these are (1) the beta-endorphin (POMC) neurons (crucial to the central effects of morphine), (2) the alpha MSH-specific system, (3) vasopressin (AVP, (4) corticotropin releasing factor (CRF), and (5) thyrotropin releasing factor (TRF). A newly developed isolation technique will be used to prepare cultures of pure populations of POMC, AVP, and CRF neurons from the hypothalami of normal and morphine-treated animals. Beta-endorphin (POMC) neurons will be studied in detail with respect to: (1) early post-translational processing, and upon acetylation of POMC peptides, particularly beta-endorphin and alpha-MSH, (2) interactions with TRF which may be an endogenous antagonist, (3) gastric, which may coexist in the POMC neuron, and (4) their (opiate) pre- and postsynaptic receptors.

吗啡对大脑多种神经内分泌功能的影响 将在本提案中进行研究。 已发现五种神经肽系统 选择的；这些是 (1) β-内啡肽 (POMC) 神经元（对于 吗啡的中枢作用），(2) α MSH 特异性系统，(3) 加压素 (AVP)、(4) 促肾上腺皮质激素释放因子 (CRF) 和 (5) 促甲状腺素释放因子（TRF）。 新开发的隔离技术 将用于制备 POMC、AVP 和 CRF 纯群体的培养物 来自正常和吗啡治疗动物下丘脑的神经元。 β-内啡肽 (POMC) 神经元将在以下方面进行详细研究： (1) 早期翻译后加工，以及 POMC 乙酰化 肽，特别是 β-内啡肽和 α-MSH，(2) 与 TRF可能是内源性拮抗剂，（3）胃，可能共存 POMC 神经元，以及 (4) 它们的（阿片类）突触前和突触后受体。