Control of dynamic palmitoylation: Identification of de-palmitoylating enzymes and their substrates in plants

动态棕榈酰化的控制：植物中去棕榈酰化酶及其底物的鉴定

基本信息

批准号：
BB/P007902/1
负责人：
Piers Hemsley
金额：
$ 46.87万
依托单位：
University of Dundee
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FP007902%2F1
关键词：
Control dynamic palmitoylation Identification de

项目摘要

Regulating protein function in plants - identification of new regulatory enzymes and their substratesProteins are the key functional molecules in the cell, forming structural building blocks and performing the many specialized functions a cell requires to survive. Making sure that individual proteins perform the right task at the right time is achieved by modifying them with various small molecules at specific places within the protein structure. This modification can alter where in the cell proteins are found, how they interact with other proteins, target them for recycling or alter how active they are. The modification of proteins that is key to this work is known as palmitoylation and involves the addition or removal of fatty acids from proteins to activate or suppress different responses. These cycles of addition and removal can promote responses through a wide range of different physical mechanisms depending on the individual protein. As these modifications are vital for regulating how a cell operates it is crucial for us to understand the mechanisms and processes that control and regulate these modifications. I have already identified and characterized the mechanisms by which palmitoyl groups are added to proteins in plants. This project aims to identify and characterize the mechanisms by which palmitoyl groups are removed from proteins in plants. This is essential for understanding the regulatory framework surrounding palmitoylation.Once we have this knowledge we will then be in a position to identify all of the proteins that are regulated by palmitoylation. This will open the door to greater understanding of how cellular function is regulated and helps the organism operate as a whole. Palmitoylation affects nearly 15% of all proteins within the cell but how it regulates their function is essentially unknown. Using the tools we have developed we will identify proteins that undergo de-palmitoylation. This will allow us to specifically study proteins regulated by changes in palmitoylation state and begin to understand how changes in palmitoylation state alter protein function.Palmitoylated proteins are involved in virtually every process in the cell that involves signaling or transport of molecules from outside of the cell to the inside. These proteins are therefore extremely important for interactions with the outside world and understanding how they are regulated to correctly perform their function will be extremely important for understanding how plants interact with their environment, fight off pathogens, build their cell walls and take up nutrients. This is in turn important for improving the food yield of crops, improving water, land and nutrient use efficiency and helping increase biofuel production - all essential parts of future food and energy security.

调节植物中的蛋白质功能 - 鉴定新的调节酶及其底层蛋白是细胞中的关键功能分子，形成结构构建块并执行细胞生存所需的许多专业功能。通过在蛋白质结构内的特定位置用各种小分子修饰它们，确保在正确的时间执行正确的任务。这种修饰可以改变在细胞蛋白中发现的地方，它们如何与其他蛋白质相互作用，靶向回收或改变其活性。这项工作关键的蛋白质的修饰称为棕榈酰化，涉及从蛋白质中添加或去除脂肪酸以激活或抑制不同的反应。这些添加和去除的周期可以通过各种不同的物理机制来促进反应，具体取决于单个蛋白质。由于这些修饰对于调节细胞的运行方式至关重要，因此对于我们了解控制和调节这些修饰的机制和过程至关重要。我已经确定并表征了将棕榈酰基添加到植物中蛋白质中的机制。该项目旨在识别和表征从植物中蛋白质中去除棕榈酰基基团的机制。这对于理解围绕棕榈酰化的调节框架至关重要。一旦我们拥有这些知识，我们将有能力识别所有受棕榈酰化调节的蛋白质。这将为更了解细胞功能的调节方式打开大门，并帮助生物体整体运作。棕榈酰化会影响细胞内所有蛋白质的近15％，但是它如何调节其功能本质上是未知的。使用我们开发的工具，我们将识别经过去皮酰化的蛋白质。这将使我们能够专门研究受棕榈酰化状态变化调节的蛋白质，并开始了解棕榈酰化状态的变化如何改变蛋白质功能。宝氨酰化的蛋白质几乎参与了细胞中的每个过程，涉及分子从细胞外部到内部的信号传递或传输。因此，这些蛋白质对于与外界的互动并了解如何正确执行其功能非常重要，这对于了解植物如何与环境相互作用，抗击病原体，建造细胞壁并吸收营养非常重要。反过来，这对于提高农作物的粮食产量，改善水，土地和营养利用效率并有助于提高生物燃料生产至关重要 - 未来食品和能源安全的所有基本部分。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

S-acylation stabilizes ligand-induced receptor kinase complex formation during plant pattern-triggered immune signalling

S-酰化在植物模式触发的免疫信号传导过程中稳定配体诱导的受体激酶复合物的形成

DOI：
10.1101/2021.08.30.457756
发表时间：
2021
期刊：
影响因子：
0
作者：
Hurst C
通讯作者：
Hurst C

Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.

DOI：
10.2144/000114516
发表时间：
2017-02-01
期刊：
BioTechniques
影响因子：
2.7
作者：
Hurst CH;Turnbull D;Plain F;Fuller W;Hemsley PA
通讯作者：
Hemsley PA

ProtView: A Versatile Tool for In Silico Protease Evaluation and Selection in a Proteomic and Proteogenomic Context.

DOI：
10.1021/acs.jproteome.3c00135
发表时间：
2023-07-07
期刊：
JOURNAL OF PROTEOME RESEARCH
影响因子：
4.4
作者：
Puliasis, Sophia S. S.;Lewandowska, Dominika;Hemsley, Piers A. A.;Zhang, Runxuan
通讯作者：
Zhang, Runxuan

An outlook on protein S-acylation in plants: what are the next steps?