Development of phloem-mobile xenobiotics with enhanced transport properties

开发具有增强运输特性的韧皮部移动异生素

• 批准号: BB/M025160/1
• 负责人: Karl Oparka
• 金额: $ 101.06万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM025160%2F1
• 关键词: Development; phloem; mobile; xenobiotics; enhanced

Every year, several thousand chemicals are screened by the agrochemical industry for potential uses as herbicides and pesticides. Very few of these compounds make it to the market place because they do not enter or move in plant tissues effectively. The ability of a given molecule to enter the phloem, the plant's internal conducting system, is a major hurdle to the development of successful systemic herbicides. The worldwide success of Glyphosate (Roundup) is due predominantly to the fact that this chemical moves extremely effectively in the phloem and is therefore able to exert its toxic effects on distant growing tissues. Unlike many other herbicides, Glyphosate appears to use plant membrane carrier proteins for its effective transport. The aim of this project is to develop new-generation chemicals that are designed to move effectively in the phloem.The run-off that accompanies field applications of chemicals is a matter of considerable environmental concern as several of these potentially toxic chemicals may pollute steams and waterways. It is clear that the development of new chemicals that reach their target sites by more efficient transport would be a major advantage in this field, reducing the amounts that need to be applied to crops and thereby minimizing run-off. This project is therefore aimed directly at improving the movement of agrochemicals in plants by the rational design of new chemicals that show enhanced phloem-mobility properties.The project takes a novel approach by using fluorescent probes that are designed specifically to move in the phloem of plants. Using fluorescence-based chemicals means that the process of uptake and transport can be studied in fine detail using appropriate fluorescence detection methods (e.g. fluorescence microscopy). A major goal is to 'piggy back' some of these fluorescent compounds into the phloem using existing proteins that function to transport natural compounds in the phloem.

每年，农化行业都会对数千种化学物质进行筛选，以确定其作为除草剂和杀虫剂的潜在用途。这些化合物很少能进入市场，因为它们不能有效地进入植物组织或在植物组织中移动。特定分子进入韧皮部的能力是开发成功的系统性除草剂的主要障碍。韧皮部是植物的内部传导系统。草甘膦(Roundup)在世界范围内的成功主要是由于这种化学物质在韧皮部中极其有效地移动，因此能够对远处生长的组织产生毒害作用。与许多其他除草剂不同，草甘膦似乎使用植物膜载体蛋白进行有效的运输。该项目的目的是开发新一代化学品，旨在有效地在韧皮部中移动。田间应用化学品时产生的径流是一个相当大的环境问题，因为其中几种潜在的有毒化学品可能会污染蒸汽和水道。显然，通过更高效的运输到达目标地点的新化学品的开发将是这一领域的一大优势，从而减少需要用于农作物的数量，从而最大限度地减少径流。因此，该项目旨在通过合理设计具有增强韧皮部流动特性的新化学物质来改善农用化学品在植物中的移动。该项目采用了一种新的方法，使用专门设计用于在植物韧皮部中移动的荧光探针。使用以荧光为基础的化学品意味着可以使用适当的荧光检测方法(例如荧光显微镜)详细研究吸收和运输过程。一个主要的目标是利用现有的蛋白质将这些荧光化合物中的一些带进韧皮部，这些蛋白质具有运输韧皮部中天然化合物的功能。

项目成果

Moderne Strategien zur Synthese funktioneller Fluorophore

• DOI: 10.1002/ange.201609394
• 发表时间: 2017-03
• 期刊: Angewandte Chemie
• 作者: F. Moliner;Nicola Kielland;R. Lavilla;M. Vendrell

A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism.

• DOI: 10.1002/anie.202016802
• 发表时间: 2021-03-29
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: de Moliner F;Knox K;Gordon D;Lee M;Tipping WJ;Geddis A;Reinders A;Ward JM;Oparka K;Vendrell M
• 通讯作者: Vendrell M

SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism

SCOTflors：用于细胞代谢体内成像的小型、可共轭、正交和可调谐荧光团

• DOI: 10.1002/ange.201900465
• 发表时间: 2019
• 期刊: Angewandte Chemie
• 作者: Benson S

The "Phagocytic Synapse" and Clearance of Apoptotic Cells.

• DOI: 10.3389/fimmu.2017.01708
• 发表时间: 2017
• 期刊: Frontiers in immunology
• 影响因子: 7.3
• 作者: Barth ND;Marwick JA;Vendrell M;Rossi AG;Dransfield I
• 通讯作者: Dransfield I