Imaging the early events of virus infection in plants

对植物中病毒感染的早期事件进行成像

• 批准号: BB/E001564/1
• 负责人: Karl Oparka
• 金额: $ 46.01万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE001564%2F1
• 关键词: Imaging; early; events; virus; infection

This project will investigate the location and redistribution of fluorescent viral RNA immediately following its microinjection into living plant cells. The project will utilise real-time imaging approaches and newly developed FlAsH and ReAsH tags to track viral gene products immediately following their translation. These tagged proteins will be imaged against a transgenic background in which a range of cellular components (e.g. ER, microtubules, plasmodesmata) have been stably highlighted with green fluorescent protein (GFP). The aim is to provide unequivocal evidence for the pathway of viral RNA trafficking in plants during the first stages of infection, and to identify the viral and host factors that interact with the viral genome during transit. The project will be carried out in collaboration with Dr Ilan Davis, Wellcome Centre for Cell Biology, and will utilise real-time deconvolution imaging approaches developed in the Central Optical Imaging Laboratory (COIL), Edinburgh University.

该项目将研究荧光病毒RNA在显微注射到活植物细胞后的位置和重新分布。该项目将利用实时成像方法和新开发的FlAsH和ReAsH标签，在病毒基因产物翻译后立即跟踪它们。这些标记的蛋白质将在转基因背景下成像，其中一系列细胞组分（例如ER、微管、胞间连丝）已经用绿色荧光蛋白（GFP）稳定地突出显示。其目的是提供明确的证据，在感染的第一阶段，在植物中的病毒RNA运输的途径，并确定在运输过程中与病毒基因组相互作用的病毒和宿主因子。该项目将与惠康细胞生物学中心的Ilan Davis博士合作进行，并将利用爱丁堡大学中央光学成像实验室（COIL）开发的实时去卷积成像方法。

项目成果

Missing links? - The connection between replication and movement of plant RNA viruses.

• DOI: 10.1016/j.coviro.2012.09.007
• 发表时间: 2012-12
• 期刊: Current opinion in virology
• 影响因子: 5.9
• 作者: J. Tilsner;K. Oparka

Tracking the green invaders: advances in imaging virus infection in plants.

追踪绿色入侵者：植物病毒感染成像的进展。

• DOI: 10.1042/bj20100372
• 发表时间: 2010
• 期刊: The Biochemical journal
• 作者: Tilsner J