14IBCAT1FEASIBILITY Discovery and development of large/diverse user-friendly panels of novel biocatalysts

将开发生化测试，以便于对酶可能转化的小范围相关化合物的10类酶中的每一类成员进行初步筛选。那些被证明能转化一种或多种此类化合物的酶将被详细描述。这项分析将采用光吸收(分光光度法)、分离(层析)和质量测定(质谱学)等技术。分析还将涉及确定某些酶是否能够区分化合物的非重叠镜像(即立体异构体)，例如，这在为制药工业生产纯化合物时至关重要。很有可能某些酶不会转化所选择的化合物。然后，这些酶将与其他化合物进行对比测试，这些化合物将通过研究相关的科学文献而得到信息。产生的结果将为生产酶的工业合作伙伴有效地选择其他酶提供信息，这些酶与那些正在进行有趣的转化的酶是互补的。这将增加所产生的进行有趣转化的酶的总数，即产生范围广泛的筛选板，因此将执行大量有趣的转化。

Biochemical tests will be developed to facilitate initial screening of members from each of 10 classes of enzyme on a small range of relevant chemical compounds which the enzymes are likely to convert. Those enzymes that are shown to convert one or more such compounds will be characterized in detail. This analysis will employ techniques that involve absorbency of light (spectrophotometry), separation (chromatography) and mass determination (mass spectrometry). Analysis will also involve determining whether certain enzymes can distinguish between non-superimposable mirror images of chemical compounds (i.e. stereoisomers), as this is of critical importance when producing pure compounds for the pharmaceutical industry, for example. It is highly likely that some of the enzymes will not convert the chosen chemical compounds. These enzymes will then be tested against other chemical compounds that will be informed by studying the relevant scientific literature. The results generated will inform the efficient selection of other enzymes, by the industrial partner producing the enzymes, which are complementary to those that are performing interesting conversions. This will increase the total number of enzymes produced that perform interesting conversions, i.e. generate wide-ranging screening panels that will therefore perform a large range of interesting conversions.