REGIONAL DEPOSITION OF INHALED PARTICLES IN MAN

将进行实验研究，以确定 人肺中支气管和肺泡气腔以及 空域尺寸对吸入颗粒物区域沉积的影响。 对于两种香烟，将进行体内颗粒沉积的测量。 吸烟者和非吸烟者，使用磷酸三苯酯的单分散气溶胶， 氧化铁，后者用99mTc或198Au标记。 同时，a 将在新鲜切除的肺上进行一系列连续测量， 成年吸烟者、非吸烟者和意外死亡的儿童，以及中空气道 从这些肺上做的模型和组织切片 测量将包括： 呼吸机械功能，气溶胶探头的气隙大小，支气管 实性肺铸型的气道形态测定， 中空气道铸型和肺切片上的肺泡空间尺寸。 在 此外，类似的技术将应用于对驴进行的测试， 涉及体内气溶胶沉积、处死、机械功能测试 和气溶胶沉积，制备肺铸型， 组织切片，并测量颗粒沉积和空气空间的大小， 他们 直接比较将在体内空域大小和那些 在铸件和截面中测量，以及空域大小和颗粒之间 证词 所有这些测试的结果将被纳入预测 沉积模型，考虑到解剖变异性，包括 与年龄、吸烟和体质因素相关的变异性。

Experimental studies will be performed to determine the range of sizes of the bronchial and alveolar airspaces in human lungs and the effects of variations in airspace dimensions on the regional deposition of inhaled particles. Measurements of in vivo particle deposition will be made, for both cigarette smokers and nonsmokers, with monodisperse aerosols of triphenyl phosphate and ferric oxide, the latter being tagged with 99mTc or 198Au. Concurrently, a series of sequential measurements will be made on the freshly excised lungs of adult smokers, nonsmokers, and children dying accidentally, and on hollow airway casts and tissue sections made from these lungs. Measurements will include: respiratory mechanical function, airspace sizes using aerosol probes, bronchial airway morphometry on solid lung casts, intrabronchial deposition patterns in hollow airway casts, and alveolar airspace dimension on lung sections. In addition, similar techniques will be applied in tests performed on donkeys, involving in vivo aerosol deposition, sacrifice, tests of mechanical function and aerosol deposition in their excised lungs, preparation of lung casts and tissue sections, and measurements of particle deposition and airspace sizes in them. Direct comparisons will be between in vivo airspace sizes and those measured in the casts and sections, and between airspace sizes and particle deposition. The results of all of these tests will be incorporated into predictive deposition models which take anatomic variability into account, including variability associated with age, cigarette smoking, and constitutional factors.