EFFECT OF ACUTE FLUORIDE DOSES ON GROWTH OF ENAMEL

急性氟化物剂量对牙釉质生长的影响

• 批准号: 3221117
• 负责人: Ziedonis Skobe
• 金额: $ 12.59万
• 依托单位: FORSYTH MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221117
• 关键词: ameloblasts; child (0-11); circadian rhythms; dental development; dosage; fluorosis; ingested fluoride therapy; mouthwash; tooth enamel; toothbrushing

This proposal is based, in part, on results of a clinical survey indicating a significant prevalence of fluorosis among children occurs in areas having non-fluoridated drinking water. The source of ingested F is not known. This proposal is directed at the possibility that fluoride is derived from the ingestion of discrete, high doses of fluoride, possibly obtained from fluoride tablets, or fluoridated tooth paste. It is very likely that the effects of such acute fluoride doses differ markedly from the chronic effects observed in areas of high fluoride concentrations in drinking water. We observed that enamel deposition follows a circadian rhythm resulting in formation of enamel of two different densities. It is very likely that there are two kinds of secretory ameloblasts, or two secretory cell cycles. This is an important finding pertaining to the basic mechanism of enamel formation. Rhythmic tooth formation has been observed in rat incisors as stripes on the tooth surface and in human enamel as incremental lines. In rats the cyclinical enamel deposition is accentuated by administration of fluoride. The purposes are to examine in detail the hypermineralized and the hypomineralized phases of the secretory, maturation, and erupted regions of the tooth with respect to: (a) the ultrastructure of enamel and enamel matrix; (b) the length of time during which each is predominant; and (c) the cytostructure of the ameloblasts secreting each phase. Secondly, to examine and measure several parameters, including fluoride serum concentrations resulting from ingesting acute fluoride doses. The goal is to investigate the mechanism causing fluorosis in order to better define optimal fluoride treatment for maximal protection without adverse effects on enamel. Thirdly, to determine whether the effect of acute fluoride doses superimposed on ambient fluoride is additive, or, as proposed here, that critical concentrations of ambient fluoride reduce or prevent fluorosis.

这项建议部分是根据一项临床调查的结果提出的 表明氟中毒在儿童中普遍存在 发生在饮用水不含氟的地区。 源 摄入的F是未知的。 这项建议是针对 氟化物来源于摄入离散的， 高剂量的氟化物，可能从氟化物片剂中获得，或 含氟牙膏 很有可能这种影响 急性氟剂量与慢性效应明显不同 在饮水氟浓度高的地区观察到 水 我们观察到釉质沉积遵循昼夜节律 导致形成两种不同密度的釉质。 是 很可能存在两种分泌型成釉细胞，或者 两个分泌细胞周期 这是一个重要的发现， 釉质形成的基本机制 节律齿 在大鼠切牙中观察到牙上的条纹 表面和人类牙釉质中的增量线。 在大鼠中， 周期性釉质沉积通过施用 氟化 目的是详细检查高矿化和 低矿化阶段的分泌，成熟， 牙齿的萌出区域关于：（a） 釉质和釉质基质的超微结构;（B）牙釉质的长度 时间期间，每一个是占主导地位的;和（c）细胞结构 分泌每一阶段的成釉细胞。 第二，检查 并测量几个参数，包括血清氟 急性氟摄入量引起的浓度。 的 目的是探讨氟中毒的发病机制， 更好地确定最佳的氟化物治疗，以获得最大的保护 对牙釉质没有不利影响。 第三，确定 急性氟剂量的影响是否叠加在 环境中的氟化物是添加剂，或者，如这里所提出的， 环境氟化物的浓度减少或防止氟中毒。