JEOL JSM-6510LV scanning electron microscope

描述（由申请人提供）：Forsyth研究所申请获得一台JEOL JSM-6510扫描电子显微镜（SEM），配备EDX Genesis能量色散x射线元素分析系统（EDS）。目的是替换20年的功能退化的扫描电镜和功能不正常的能谱仪，并获得新技术。扫描电镜的新技术允许在低电压下进行组织检查，而不会立即使样品干燥和烘烤，导致收缩和开裂。新的背散射电子探测器技术在过去的20年里有了很大的进步。现在可以检查没有金属涂层的样品，这会干扰免疫金标记和元素分析，并且在VP模式下，没有完全干燥。JSM-6510采用新的软件，便于操作和数据收集。我们目前的扫描电镜每周使用大约25个小时，在邻近的机构没有新的扫描电镜或WDS，我们可以使用或租用这段时间。除了分析来自13个NIH支持和提交的Forsyth项目的样本外，SEM已经并将成为我们矿化组织分析中心的主要仪器，用于检查NIH资助的范德比尔特、洛克菲勒、德克萨斯A&M大学、哈佛大学和波士顿大学牙科医学院、NIDCR和波士顿儿童医院的研究项目中的敲除小鼠牙齿。研究者和共同研究者都是具有多年经验的SEM专家，并发表了许多SEM论文。Forsyth SEM委员会将监督维护并执行保护操作人员和SEM免受伤害的政策。通过远程访问的电子房间建立了日程安排。福赛斯研究所保证提供：扫描电镜操作技术人员；对SEM的住房进行翻新；服务合同;升级;培训;和维护。福赛斯是世界上最重要的研究机构，专注于口腔健康、矿化组织研究和颅面发育生物学。为了保持高水准和招募新的科学家，必须随时提供尖端的仪器。扫描电镜和EDS系统将极大地有利于福赛斯的研究，旨在了解和根除颅面和牙齿畸形的遗传基础。扫描电镜将用于研究口腔细菌感染以及如何改善治疗和预防。扫描电镜对矿化组织的研究至关重要，并有助于开发修复和恢复病变或受损组织的新方法。

DESCRIPTION (provided by applicant): The Forsyth Institute proposes to obtain a JEOL JSM-6510 scanning electron microscope (SEM) equipped with an EDX Genesis energy dispersive x-ray elemental analysis system (EDS). The purpose is to replace a 20-year-old SEM with deteriorating functions and a non-functioning EDS and to acquire new technology. The new technology of the SEM allows tissue examination at low voltages that do not immediately desiccate and bake the samples causing shrinkage and cracking. New backscattered electron detector technology is vastly improved over the past 20 years. Now it is possible to examine samples without metal coating, which interferes with immunogold labeling and elemental analysis, and in the VP mode, without complete drying. The JSM-6510 has new software that permits ease of operation and data collection. Our current SEM is used about 25 hours per week, there is no new SEM or WDS at neighboring institutions that we could use or rent this amount of time. In addition to analyzing samples from thirteen NIH-supported and submitted projects at Forsyth, the SEM has been, and will be the major instrument in our mineralized tissue analysis center used to examine knock out mice teeth from NIH sponsored research projects at Vanderbilt, Rockefeller, Texas A&M Universities, and Harvard and Boston University Schools of Dental Medicine, NIDCR, and Children's Hospital in Boston. The investigator and co-investigator are expert SEM operators with years of experience and have many SEM publications. The Forsyth SEM Committee will monitor maintenance and enforce policies for protecting the operators and the SEM from harm. Scheduling has been established via an e-room with remote access. The Forsyth Institute guarantees to provide: technician for operation of the SEM; renovations for housing the SEM; service contracts; upgrades; training; and maintenance. Forsyth is the world's foremost research institute focusing on oral health, mineralized tissue research, and craniofacial developmental biology. In order to maintain high standards and recruit new scientists, cutting- edge instrumentation must be readily available. The SEM and EDS system will greatly benefit research at Forsyth aimed at understanding and eradicating genetic bases of craniofacial and dental malformations. The SEM will be used to study oral bacterial infections and how to improve treatment and prevention. The SEM will be essential for studies of mineralized tissues and aid in the development of new approaches of repair and restoration of diseased or damaged tissues.