JEOL JSM-6510LV scanning electron microscope

描述(由申请人提供)：福赛斯研究所建议获得一台JEOL JSM-6510扫描电子显微镜(SEM)，配备EDX Genesis能量色散X射线元素分析系统(EDS)。其目的是更换功能日益恶化的已有20年历史的扫描电子显微镜和不起作用的EDS，并获得新技术。扫描电子显微镜的新技术允许在低电压下进行组织检查，而不会立即干燥和烘烤导致收缩和破裂的样品。在过去的20年里，新的背散射电子探测器技术得到了极大的改进。现在可以在没有金属涂层的情况下检查样品，这会干扰免疫金标记和元素分析，并且可以在VP模式下进行检查，而不需要完全干燥。JSM-6510拥有新的软件，使操作和数据收集变得容易。我们目前的扫描电子显微镜每周大约使用25个小时，邻近机构没有新的扫描电子显微镜或WDS可以使用或租用这段时间。除了分析NIH支持并在Forsyth提交的13个项目的样本外，扫描电子显微镜已经并将成为我们矿化组织分析中心的主要工具，用于检查NIH赞助的范德比尔特大学、洛克菲勒大学、德克萨斯农工大学、哈佛大学和波士顿大学牙科医学院、NIDCR和波士顿儿童医院的研究项目中敲除的老鼠牙齿。调查员和联合调查员都是具有多年经验的专家扫描电子显微镜操作员，并有许多扫描电子显微镜出版物。Forsyth SEM委员会将监督维护并执行保护操作员和SEM免受伤害的政策。通过远程访问的电子会议室建立了日程安排。福赛斯研究所保证提供：操作扫描电子显微镜的技术人员；安装扫描电子显微镜的翻新；服务合同；升级；培训和维护。Forsyth是世界上最顶尖的研究机构，专注于口腔健康、矿化组织研究和颅面发育生物学。为了保持高标准和招募新的科学家，尖端仪器必须随时可用。扫描电子显微镜和能谱分析系统将极大地促进福赛斯的研究，旨在了解和根除颅面和牙齿畸形的遗传基础。扫描电子显微镜将用于研究口腔细菌感染以及如何改进治疗和预防。扫描电子显微镜将对矿化组织的研究至关重要，并有助于开发新的修复和恢复患病或受损组织的方法。

DESCRIPTION (provided by applicant): The Forsyth Institute proposes to obtain a JEOL JSM-6510 scanning electron microscope (SEM) equipped with an EDX Genesis energy dispersive x-ray elemental analysis system (EDS). The purpose is to replace a 20-year-old SEM with deteriorating functions and a non-functioning EDS and to acquire new technology. The new technology of the SEM allows tissue examination at low voltages that do not immediately desiccate and bake the samples causing shrinkage and cracking. New backscattered electron detector technology is vastly improved over the past 20 years. Now it is possible to examine samples without metal coating, which interferes with immunogold labeling and elemental analysis, and in the VP mode, without complete drying. The JSM-6510 has new software that permits ease of operation and data collection. Our current SEM is used about 25 hours per week, there is no new SEM or WDS at neighboring institutions that we could use or rent this amount of time. In addition to analyzing samples from thirteen NIH-supported and submitted projects at Forsyth, the SEM has been, and will be the major instrument in our mineralized tissue analysis center used to examine knock out mice teeth from NIH sponsored research projects at Vanderbilt, Rockefeller, Texas A&M Universities, and Harvard and Boston University Schools of Dental Medicine, NIDCR, and Children's Hospital in Boston. The investigator and co-investigator are expert SEM operators with years of experience and have many SEM publications. The Forsyth SEM Committee will monitor maintenance and enforce policies for protecting the operators and the SEM from harm. Scheduling has been established via an e-room with remote access. The Forsyth Institute guarantees to provide: technician for operation of the SEM; renovations for housing the SEM; service contracts; upgrades; training; and maintenance. Forsyth is the world's foremost research institute focusing on oral health, mineralized tissue research, and craniofacial developmental biology. In order to maintain high standards and recruit new scientists, cutting- edge instrumentation must be readily available. The SEM and EDS system will greatly benefit research at Forsyth aimed at understanding and eradicating genetic bases of craniofacial and dental malformations. The SEM will be used to study oral bacterial infections and how to improve treatment and prevention. The SEM will be essential for studies of mineralized tissues and aid in the development of new approaches of repair and restoration of diseased or damaged tissues.