LEICS TCS SP2 CONFOCAL LASER SCANNING MICROSCOPE

LEICS TCS SP2 共焦激光扫描显微镜

• 批准号: 6288096
• 负责人: Ziedonis Skobe
• 金额: $ 31.23万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288096
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

This proposal is for the Biostructure Core Facility of The Forsyth Institute to obtain a Leica TCS SP2 Confocal Laser Scanning head mounted on an upright DM RXE microscope (CLSM) with a series of water, air, and oil objectives, fired with Ar ion and two He/Ne lasers. A satellite image analysis center is included. Forsyth is the worlds foremost independent research institute in oral and craniofacial biology. In order to maintain high standards of achievement it is time to expand existing instrumentation. Several of the grantees currently outsource time on different CLSMs, two grants depend on CLSM use, and two have recent publications using CLSM. Obtaining a CLSM ideally enhances four broad scientific interests at Forsyth. (1) Several investigators at Forsyth are studying oral biofilm formation, the interaction of biofilm with epithelial cells, or the immune response to biofilm. The CLSM is the instrument of choice for the study of the bacterial composition of biofilm. (2) Others at Forsyth are studying specific gene expression, protein expression and molecular interactions in craniofacial mutations occurring in humans. The Zebrafish facility at Forsyth is a source of embryos used to study genes and proteins involved in normal and mutant craniofacial cartilage differentiation. The optical sectioning capability of the CLSM is ideal for locating fluoroprobes used to mark genes and proteins in cells and tissues. (3) Basic research is conducted at Forsyth in the interaction of cell adhesion molecules, the cytoskeleton, and enamel matrix proteins in biomineralization. (4) Another focal interest is exploring mechanisms of gene regulation in bone resorption by localizing gene expression, protein elaboration and molecular interactions in osteoclasts. The CLSM is designed to accommodate techniques that address these research approaches. Use of the CLSM will be shared among more than 12 investigators, their post-doctoral fellows, and scientists from Northeastern University and Harvard School of Dental Medicine. The Forsyth CLSM Oversight Committee will regulate all aspects of CLSM use. The Forsyth Institute guarantees to provide: 1) all necessary renovations for housing the CLSM, 2) the service contract for the life of the instrument, 3) applications support, and 4) a trained operator to be available for all users of the CLSM.

该提案旨在为福赛斯研究所的生物结构核心设施提供安装在直立 DM RXE 显微镜 (CLSM) 上的 Leica TCS SP2 共焦激光扫描头，并配有一系列水、空气和油物镜，用 Ar 离子和两个 He/Ne 激光器发射。包括一个卫星图像分析中心。福赛斯是世界上最重要的口腔和颅面生物学独立研究机构。为了保持高标准的成就，是时候扩展现有仪器了。目前，一些受资助者在不同的 CLSM 上外包时间，两项资助取决于 CLSM 的使用，两项资助最近发表了使用 CLSM 的出版物。获得 CLSM 可以理想地增强福赛思的四大科学兴趣。 (1) 福赛思的几位研究人员正在研究口腔生物膜的形成、生物膜与上皮细胞的相互作用或对生物膜的免疫反应。 CLSM 是研究生物膜细菌组成的首选仪器。 (2) 福赛斯的其他人正在研究人类颅面突变中的特定基因表达、蛋白质表达和分子相互作用。福赛斯的斑马鱼设施是用于研究参与正常和突变颅面软骨分化的基因和蛋白质的胚胎来源。 CLSM 的光学切片功能非常适合定位用于标记细胞和组织中的基因和蛋白质的荧光探针。 (3) Forsyth 进行细胞粘附分子、细胞骨架和牙釉质基质蛋白在生物矿化过程中相互作用的基础研究。 (4) 另一个焦点是通过定位破骨细胞中的基因表达、蛋白质加工和分子相互作用来探索骨吸收中的基因调控机制。 CLSM 旨在适应解决这些研究方法的技术。 CLSM 的使用将由超过 12 名研究人员、他们的博士后研究员以及来自东北大学和哈佛大学牙科医学院的科学家共享。 Forsyth CLSM 监督委员会将监管 CLSM 使用的各个方面。福赛思研究所保证提供：1) CLSM 外壳所需的所有翻新，2) 仪器使用寿命内的服务合同，3) 应用支持，以及 4) 为 CLSM 的所有用户提供经过培训的操作员。