LEICS TCS SP2 CONFOCAL LASER SCANNING MICROSCOPE

这项提议是为了让福赛斯研究所的生物结构核心设施获得徕卡TCS SP2共聚焦激光扫描头，该扫描头安装在立式DM RXE显微镜(CLSM)上，带有一系列水、空气和石油目标，使用Ar离子和两个He/Ne激光进行发射。包括一个卫星图像分析中心。福赛斯是世界上口腔和颅面生物学领域最领先的独立研究机构。为了保持高标准的成就，现在是扩大现有手段的时候了。几个受赠人目前将时间外包给不同的CLSM，两个赠款取决于CLSM的使用，两个最近出版了使用CLSM的出版物。在理想情况下，获得CLSM学位可以增强福赛斯的四大科学兴趣。(1)Forsyth的几名研究人员正在研究口腔生物膜的形成，生物膜与上皮细胞的相互作用，或对生物膜的免疫反应。激光共聚焦显微镜是研究生物膜细菌组成的首选仪器。(2)Forsyth的其他研究人员正在研究人类头面部突变中的特定基因表达、蛋白质表达和分子相互作用。位于Forsyth的斑马鱼设施是一个胚胎来源，用于研究与正常和突变的头面部软骨分化有关的基因和蛋白质。CLSM的光学切片能力是定位用于标记细胞和组织中的基因和蛋白质的荧光探针的理想选择。(3)在福赛斯进行了细胞黏附分子、细胞骨架和釉质基质蛋白在生物矿化过程中相互作用的基础研究。(4)通过对破骨细胞基因表达、蛋白质合成和分子相互作用的定位，探讨基因调控骨吸收的机制。CLSM是为适应解决这些研究方法的技术而设计的。超过12名研究人员、他们的博士后研究员以及来自东北大学和哈佛大学牙医学院的科学家将共享CLSM的使用。福赛斯CLSM监督委员会将规范CLSM使用的方方面面。福赛斯研究所保证：1)为安装CLSM提供所有必要的翻新；2)签订仪器使用寿命的服务合同；3)提供应用支持；4)为CLSM的所有用户提供训练有素的操作员。

This proposal is for the Biostructure Core Facility of The Forsyth Institute to obtain a Leica TCS SP2 Confocal Laser Scanning head mounted on an upright DM RXE microscope (CLSM) with a series of water, air, and oil objectives, fired with Ar ion and two He/Ne lasers. A satellite image analysis center is included. Forsyth is the worlds foremost independent research institute in oral and craniofacial biology. In order to maintain high standards of achievement it is time to expand existing instrumentation. Several of the grantees currently outsource time on different CLSMs, two grants depend on CLSM use, and two have recent publications using CLSM. Obtaining a CLSM ideally enhances four broad scientific interests at Forsyth. (1) Several investigators at Forsyth are studying oral biofilm formation, the interaction of biofilm with epithelial cells, or the immune response to biofilm. The CLSM is the instrument of choice for the study of the bacterial composition of biofilm. (2) Others at Forsyth are studying specific gene expression, protein expression and molecular interactions in craniofacial mutations occurring in humans. The Zebrafish facility at Forsyth is a source of embryos used to study genes and proteins involved in normal and mutant craniofacial cartilage differentiation. The optical sectioning capability of the CLSM is ideal for locating fluoroprobes used to mark genes and proteins in cells and tissues. (3) Basic research is conducted at Forsyth in the interaction of cell adhesion molecules, the cytoskeleton, and enamel matrix proteins in biomineralization. (4) Another focal interest is exploring mechanisms of gene regulation in bone resorption by localizing gene expression, protein elaboration and molecular interactions in osteoclasts. The CLSM is designed to accommodate techniques that address these research approaches. Use of the CLSM will be shared among more than 12 investigators, their post-doctoral fellows, and scientists from Northeastern University and Harvard School of Dental Medicine. The Forsyth CLSM Oversight Committee will regulate all aspects of CLSM use. The Forsyth Institute guarantees to provide: 1) all necessary renovations for housing the CLSM, 2) the service contract for the life of the instrument, 3) applications support, and 4) a trained operator to be available for all users of the CLSM.