STRUCTURES AND MECHANISMS OF CITRATE ENZYMES

我们将继续对其结构、机制和 柠檬酸裂解酶、柠檬酸三种酶的生物学功能 合酶和ATP-柠檬酸裂解酶。在这项提案中，我们打算研究 柠檬酸合酶的原位结构与其结构变化的关系 一种三磷酸腺苷敏感酶对三磷酸腺苷的抑制模式 在活体内不敏感的。我们还将尝试确定这些基因是否 对于柠檬酸裂解酶都是由一个载体携带的，并试图分离和研究 这些基因。最后，我们将研究残基和周围的多肽。 在负责形成组氨酸的ATP-柠檬酸裂解酶中 磷酸盐中间体和柠檬基酶中间体 反应。我们将研究最近纯化的大鼠脑的性质 ATP-柠檬酸裂解酶。我们将继续努力将这些机制联系起来。 以及三种柠檬酸盐酶的结构。此外，我们还将使用 关于酶的结构和机制的信息，以深入了解每种酶 酶的生物学作用及其活性的控制。

We will continue our investigations on the structure, mechanism and biological functions of the three citrate enzymes, citrate lyase, citrate synthase and ATP-citrate lyase. In this proposal we intend to examine the structure of citrate synthase in situ in relation to the change in its inhibitory pattern from an ATP sensitive enzyme in vitro to an ATP insensitive one in vivo. We shall also attempt to determine if the genes for citrate lyase are carried by a plasmid and attempt to isolate and study these genes. Finally, we shall study the residues and surrounding peptides in ATP-citrate lyase that are responsible for the formation of a histidyl phosphate intermediate and the citryl enzyme intermediate in the overall reaction. We will study the properties of the recently purified rat brain ATP-citrate lyase. We will continue our efforts to relate the mechanisms and structures of the three citrate enzymes. In addition, we will use the information on enzyme structure and mechanism to gain insight into each enzymes biological role and the control of its activity.