14 NSFBIO: Asymmetric division and the temporal dynamics of cell motility

14 NSFBIO：不对称分裂和细胞运动的时间动态

• 批准号: BB/N013174/1
• 负责人: Shane Herbert
• 金额: $ 35.56万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN013174%2F1
• 关键词: 14; NSFBIO; Asymmetric; division; temporal

This project aims to define the fundamental mechanisms controlling cell migration during organ development. In growing tissues, cells usually replicate by symmetric division (or mitosis) to produce two identical daughter cells. But in some instances cell divisions are asymmetric and give rise to intrinsically distinct daughters. Asymmetric divisions play important roles in differential cell fate decisions, but have never previously been implicated in generating daughters with distinct motilities. This project will explore the molecular and cellular basis of post-mitotic asymmetry in cell motility and define the functional role of asymmetric divisions in the control of cell migration during tissue growth. In particular this work will evaluate how asymmetries is daughter cell signaling, shape and stiffness can profoundly affect cell motility following division. Not only will this research generate freely available novel computational methods and analysis tools suitable for a wide array of applications, the findings of this work will have wide-reaching implications for understanding the control of cell migration across a plethora of cellular systems and organisms. The project will additionally expose undergraduate and high school students (with emphasis on recruiting underrepresented groups) to integrated cross-disciplinary computational / experimental scientific approaches and provide hands-on experience of international collaborative research techniques upon the creation of several new, targeted, interactive Global Interface Science (GIS) workshops. Moreover, guidance on implementing similar GIS workshops anywhere worldwide will be widely disseminated via online media.

该项目旨在定义器官发育过程中控制细胞迁移的基本机制。在生长的组织中，细胞通常通过对称分裂（或有丝分裂）进行复制，产生两个相同的子细胞。但在某些情况下，细胞分裂是不对称的，并产生本质上不同的子细胞。不对称分裂在差异细胞命运决定中发挥着重要作用，但以前从未涉及产生具有不同运动能力的子细胞。该项目将探索细胞运动中有丝分裂后不对称性的分子和细胞基础，并定义不对称分裂在组织生长过程中细胞迁移控制中的功能作用。特别是，这项工作将评估子细胞信号传导、形状和硬度的不对称性如何深刻影响分裂后的细胞运动。这项研究不仅将产生适合广泛应用的免费新型计算方法和分析工具，而且这项工作的发现将对理解细胞在众多细胞系统和生物体中迁移的控制产生广泛的影响。该项目还将让本科生和高中生（重点是招募代表性不足的群体）接触综合的跨学科计算/实验科学方法，并在创建几个新的、有针对性的、交互式全球界面科学（GIS）研讨会时提供国际合作研究技术的实践经验。此外，关于在世界各地举办类似 GIS 研讨会的指南将通过在线媒体广泛传播。

项目成果

MEF2 transcription factors are key regulators of sprouting angiogenesis.

• DOI: 10.1101/gad.290619.116
• 发表时间: 2016-10-15
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Sacilotto N;Chouliaras KM;Nikitenko LL;Lu YW;Fritzsche M;Wallace MD;Nornes S;García-Moreno F;Payne S;Bridges E;Liu K;Biggs D;Ratnayaka I;Herbert SP;Molnár Z;Harris AL;Davies B;Bond GL;Bou-Gharios G;Schwarz JJ;De Val S
• 通讯作者: De Val S

Asymmetric division coordinates collective cell migration in angiogenesis.

• DOI: 10.1038/ncb3443
• 发表时间: 2016-12
• 期刊: Nature cell biology
• 影响因子: 21.3
• 作者: Costa G;Harrington KI;Lovegrove HE;Page DJ;Chakravartula S;Bentley K;Herbert SP
• 通讯作者: Herbert SP