BIOCHEMICAL STUDIES OF MINERALIZED TISSUE PROTEOGLYCANS

矿化组织蛋白聚糖的生物化学研究

• 批准号: 3220235
• 负责人: CHARLES W PRINCE
• 金额: $ 9.89万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220235
• 关键词: 1,25 dihydroxycholecalciferol; bone metabolism; calcification; cell bank /registry; chemical structure function; connective tissue metabolism; dentin; glycoproteins; laboratory rabbit; laboratory rat; macromolecule; normal ossification; proteoglycan; proteolysis; radiotracer; retinoids; sialate

The major hypothesis upon which this research is based is that non-collagenous components of mineralized connective tissues are intimately involved in the formation of the tissues. Furthermore, an understanding of the chemical composition, localization and regulatory mechanisms of biosynthesis are required before specific, functional roles can be elucidated. In this proposal we will address the aforementioned areas as they relate to proteoglycans and osteopontin, newly discovered bone-derived cell attachment protein. The approach taken is to isolate chemical amounts of these components, verify their purity and use them as antigens. The antibodies thus produced will be used in immunolocalization studies and as reagents in immunoassays. We will explore in detail the kinetics of proteoglycan biosynthesis in rat calvaria and long bone, in ROS 17/2.8 cells and in cultures of collagenase-derived rat calvarial osteoblasts. We will evaluate the role and mechanism that 1,25-dihydroxyvitamin D3 and parathyroid may have in the regulation of proteoglycan and osteopontin biosynthesis. We will also further explore the mechanism of action by which osteopontin stimulates cells to attach in vitro.

这项研究所基于的主要假设是 矿化结缔组织的非胶原性成分为 与组织的形成密切相关。此外， 对其化学成分、定位和性能的认识 需要生物合成的调节机制才能 具体的、功能性的角色可以被阐明。在这份提案中，我们 我将讨论上述领域，因为它们与 新发现的骨源性细胞--蛋白多糖和骨桥蛋白 附着蛋白。所采取的方法是分离化学物质 这些成分的数量，验证它们的纯度并将其用作 抗原。由此产生的抗体将用于 免疫定位研究和免疫分析中的试剂。我们 将详细探索蛋白多糖生物合成的动力学。 大鼠颅骨和长骨，在ROS 17/2.8细胞和在 胶原酶来源的大鼠颅骨成骨细胞。我们将评估 1，25-二羟基维生素D3和维生素D3的作用及机制 甲状旁腺可能在调节蛋白多糖和 骨桥蛋白的生物合成。我们还将进一步探索 骨桥蛋白刺激细胞的作用机制 在体外附着。