SALIVARY IMMUNE RESPONSE TO COMMENSAL ORAL BACTERIA

对口腔共生细菌的唾液免疫反应

• 批准号: 3221956
• 负责人: MICHAEL F. COLE
• 金额: $ 13.98万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221956
• 关键词: Actinomyces; Bacteroides; Streptococcus mutans; antibacterial antibody; bactericidal immunity; enzyme linked immunosorbent assay; genetic strain; high performance liquid chromatography; host organism interaction; immunoregulation; infant human (0-1 year); laboratory mouse; longitudinal human study; microorganism culture; monoclonal antibody; oral bacteria; preschool child (1-5); saliva

The goal of this proposal is to determine the salivary immune response to selected commensal oral bacteria. The majority of diseases that afflict man are caused by exogenous pathogens. However, the principal oral diseases, dental caries and periodontal disease, are caused be endogenous bacteria that are members of the normal oral flora. Although it can be shown that the salivary immune system recognizes and responds to oral bacteria the extent to which the immune system plays a role in the regulation of the indigenous oral flora is unclear. An understanding of the mechanisms by which host protection against cariogenic and periodontopathic bacteria is mediated is essential in the development of vaccines to control these opportunistically pathogenic commensal bacteria. The bacteria chosen for study are Streptococcus mutants, Actinomyces viscosus and A. naeslundii and Bacteroides intermedius. These bacteria were selected because a) they have been implicated in coronal and root surface caries and periodontal disease and b) they represent genera that are either unique to the mouth or are oral species of general that predominate at other mucosal surfaces of the body. It is proposed to conduct a longitudinal study in infants from birth to age two years. The specific aims of this study are to 1) determine the kinetics of colonization of S. muttons, A. viscosus, A. naeslundii and B. intermedius using selective media and fluorescent antibodies, 2) determine the levels and isotypes of salivary antibodies induced in response to the colonization of the selected bacteria using an enzyme-linked immunosorbent assay (ELISA), 3) determine the specificity of antibodies induced in response to colonization by Western blot analysis 4) identify and characterize immunodominant surface antigens by use of monoclonal antibodies and high performance liquid chromatography and 5) correlate the levels, isotypes and specificity of salivary antibodies with colonization of the selected bacteria by comparing ELISA titers and Western blot profiles with the appearance and concentration of the selected bacteria in the mouth.

这项建议的目的是确定唾液免疫反应， 选择的口腔细菌。 大多数折磨人的疾病 人是由外源性病原体引起的。 然而，主要的口头 龋齿和牙周病是由内源性疾病引起的 作为正常口腔植物群成员的细菌。 虽然可以 表明唾液免疫系统识别并响应口腔 细菌的免疫系统在多大程度上发挥作用， 本地口腔植物群的调节尚不清楚。 了解 宿主对致龋和 牙周病细菌是介导的是必不可少的发展， 疫苗来控制这些机会致病性肠道细菌。 选择用于研究的细菌是变异链球菌、放线菌 viscosus和A. naeslundii和中间拟杆菌。 这些细菌 被选中是因为a）它们与冠状和根部有关 和B）它们代表的属 要么是口腔特有的，要么是一般的口腔物种， 在身体的其他粘膜表面占优势。 提出要 对婴儿从出生到两岁进行纵向研究。 的 本研究的具体目的是：1）确定 S. muttons，A. viscosus、A. naeslundii和B. intermedius 使用选择性培养基和荧光抗体，2）测定 和唾液抗体的同种型诱导的殖民化 使用酶联免疫吸附测定法 （ELISA），3）确定响应于 通过Western印迹分析定殖4）鉴定和表征 通过使用单克隆抗体和高免疫显性表面抗原 高效液相色谱法和5）关联水平，同种型和 唾液抗体的特异性与选定的 通过比较ELISA滴度和蛋白质印迹图谱， 口腔中所选细菌的外观和浓度。