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Development of a graphene oxide nanosensor for fast detection of mRNA-related sequences in crops.

开发氧化石墨烯纳米传感器，用于快速检测农作物中的 mRNA 相关序列。

基本信息

批准号：
BB/N021150/1
负责人：
Antonios Kanaras
金额：
$ 19.25万
依托单位：
University of Southampton
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FN021150%2F1
关键词：
Development graphene oxide nanosensor fast

项目摘要

This project exploits the use of nanotechnology to design a system that will have uses in crop science and also has potential for wider applications. Nanotechnology is the engineering of functional systems at the molecular level and this project aims to develop a sensor that can be used as a tool to improve key aspects of crop research. The sensor that we will develop is based on the use of nanoparticles and graphene oxide. Graphene itself is an atom thick, hexagonal lattice, nanomaterial made of carbon atoms and graphene oxide has a number of useful properties that are amenable to its development as a biosensor. Our aim is to develop a sensor that will efficiently detect changes in gene-expression levels of various nutrient transporters in response to micronutrient stress in crops. Insufficient amounts of micronutrients such as zinc (Zn) in crop tissues has a detrimental effect on crop yield and on grain nutritional quality thus impacting food security. Membrane transporter proteins are responsible for the transport of key micronutrients in plants and under nutrient stress conditions their gene expression can be up-regulated to ensure more efficient uptake and use of the available nutrients. Our sensor is based on detecting these changes in gene expression by a process involving binding to specific oligonucleotides for the gene in question. Nucleotides serve as the basis of nucleic acids like DNA and RNA and our sensor will be designed to monitor changes in the levels of the RNA that encodes these nutrient transporters. We will focus on barley and wheat cereal crops where we have shown that under Zn stress particular members of the ZIP transporter family are up-regulated. These Zn-transporting ZIPs are proposed to increase the efficiency of Zn uptake and allocation under conditions of low Zn supply and thus can be used as markers of nutritional stress. Currently, gene expression analysis makes use of PCR-based techniques, which are time consuming and can be costly and technically challenging. A kit enabling rapid detection of nutrient deficiencies in crops by monitoring changes in marker gene expression will improve fundamental plant research and enable real world applications in crop-health monitoring.This project idea is timely for future applications and aligned with the urgency for the improved nutrition value and performance of crops across the globe. This is the first time that this newly discovered sensor based on highly advanced nanotechnological developments will be applied in crop science.

该项目利用纳米技术设计一个系统，该系统将用于作物科学，并具有更广泛的应用潜力。纳米技术是分子水平上的功能系统工程，该项目旨在开发一种传感器，可用作改善作物研究关键方面的工具。我们将开发的传感器是基于纳米颗粒和氧化石墨烯的使用。石墨烯本身是一种原子厚的六方晶格，由碳原子和氧化石墨烯制成的纳米材料，具有许多有用的特性，适合作为生物传感器开发。我们的目标是开发一种传感器，将有效地检测各种营养转运蛋白的基因表达水平的变化，以应对作物中的微量营养素胁迫。作物组织中微量营养素如锌（Zn）的量不足对作物产量和谷物营养品质具有不利影响，从而影响粮食安全。膜转运蛋白负责植物中关键微量营养素的转运，并且在营养胁迫条件下，它们的基因表达可以上调，以确保更有效地吸收和利用可用营养素。我们的传感器是基于检测基因表达的这些变化，通过一个过程，涉及结合到特定的寡核苷酸的基因问题。核苷酸作为DNA和RNA等核酸的基础，我们的传感器将被设计用于监测编码这些营养转运蛋白的RNA水平的变化。我们将集中在大麦和小麦谷类作物，我们已经表明，锌胁迫下的ZIP转运蛋白家族的特定成员上调。这些锌转运ZIPs被认为在低锌供应条件下提高了锌的吸收和分配效率，因此可以用作营养胁迫的标志物。目前，基因表达分析利用基于PCR的技术，这是耗时的，可能是昂贵的和技术上的挑战。通过监测标记基因表达的变化，能够快速检测作物营养缺乏的试剂盒将改善基础植物研究，并使作物健康监测的真实的世界应用成为可能。该项目的想法对于未来的应用是及时的，并与提高地球仪作物营养价值和性能的紧迫性相一致。这是第一次，这种基于高度先进的纳米技术发展的新发现的传感器将应用于作物科学。