CHEM. NAT. CA-P MIN. & 3-D TO COLLAGEN-CEMENTUM

这里提出的研究项目旨在确定 矿物钙磷酸盐相(S)的化学性质及其意义 胶原蛋白原纤维的三维结构关系 牙骨质。拟议的研究将描述这些特性，并致力于 相当大的努力来理解化学和结构的变化 组织成熟和老化的一种功能。 牙骨质的两种主要胞外成分是无机的。矿物 固体和有机胶原纤维，占超过80%的 组织块。这些成分的化学和结构变化，这 在组织成熟过程中发生在分子水平上，影响 组织功能的质量(即结构-机械支撑)。 目前，文献中很少有关于 牙骨质中的矿物组成及其与胶原蛋白的关系 衰老的问题。这些属性的描述，以及在老化过程中的变化 在病理情况下，将提供关键信息 将支持开发新的临床治疗方法和材料 牙周病--一个日益严重的健康问题 牙科，特别是针对日益增长的老年人口群体。 拟议的研究将广泛使用(1)高压电子 用于化学分析的显微衍射/探针微量分析；(2) 高压体视显微镜与三维计算机序列重建 超微结构表征：(3)标本制备，无水 将使用技术和超冷冻显微手术来绕过 制备和分析过程中的矿物人工制品。这些技术 代表了当今可用的最强大的工具，并结合 应该对牙骨质矿物质和胶原蛋白有最深入的了解 还没有拿到。

The research project proposed here is designed to determine the precise chemical nature of the mineral calcium phosphate phase(s) and its three-dimensional structural relationship with the collagen fibrils in cementum. The proposed study will characterize these properties and devote considerable effort to understanding the chemical and structural changes as a function of tissue maturation and aging. Two primary extracellular components of cementum are inorganic. mineral solids and organic collagen fibrils, making up over 80 percent of the tissue mass. Chemical and structural changes of these components, which take place at the molecular level during tissue maturation, influence the quality of the tissue function (i.e., structural-mechanical support). Currently, there is very little information in the literature on the mineral composition and its relations to collagen in cementum as a function of aging. The delineation of these properties, and changes during aging and under pathological conditions, will provide critical information that will support the development of new clinical treatments and materials for periodontal diseases - an increasing health problem in the field of dentistry, particularly for the growing segment of the older population. The proposed study will make extensive use of (1) high-voltage electron microdiffraction/probe microanalysis for chemical analysis; (2) high-voltage stereomicroscopy and 3-D computer serial reconstruction for ultrastructural characterization: (3) for specimen preparation, anhydrous techniques and ultracryomicrotomy will be employed in order to circumvent mineral artifacts during preparation and analyses. These techniques represent the most powerful tools available today, and in combination should yield the most intimate knowledge of cementum minerals and collagen yet obtained.