CHEM. NAT. CA-P MIN. & 3-D TO COLLAGEN-CEMENTUM

化学。

• 批准号: 3223613
• 负责人: DOSUK D LEE
• 金额: $ 19.59万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1995-05-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3223613
• 关键词: X ray crystallography; adolescence (12-20); aging; apatites; calcium phosphate; cementum; chemical structure function; collagen; computer program /software; extracellular matrix; high voltage electron microscopy; human middle age (35-64); human old age (65+); human tissue; periodontium disorder; phase change; tooth; young adult human (21-34)

The research project proposed here is designed to determine the precise chemical nature of the mineral calcium phosphate phase(s) and its three-dimensional structural relationship with the collagen fibrils in cementum. The proposed study will characterize these properties and devote considerable effort to understanding the chemical and structural changes as a function of tissue maturation and aging. Two primary extracellular components of cementum are inorganic. mineral solids and organic collagen fibrils, making up over 80 percent of the tissue mass. Chemical and structural changes of these components, which take place at the molecular level during tissue maturation, influence the quality of the tissue function (i.e., structural-mechanical support). Currently, there is very little information in the literature on the mineral composition and its relations to collagen in cementum as a function of aging. The delineation of these properties, and changes during aging and under pathological conditions, will provide critical information that will support the development of new clinical treatments and materials for periodontal diseases - an increasing health problem in the field of dentistry, particularly for the growing segment of the older population. The proposed study will make extensive use of (1) high-voltage electron microdiffraction/probe microanalysis for chemical analysis; (2) high-voltage stereomicroscopy and 3-D computer serial reconstruction for ultrastructural characterization: (3) for specimen preparation, anhydrous techniques and ultracryomicrotomy will be employed in order to circumvent mineral artifacts during preparation and analyses. These techniques represent the most powerful tools available today, and in combination should yield the most intimate knowledge of cementum minerals and collagen yet obtained.

这里提出的研究项目旨在确定精确的 矿物磷酸钙相的化学性质及其 与胶原纤维的三维结构关系 牙骨质。 拟议的研究将描述这些属性，并致力于 为了理解化学和结构变化， 组织成熟和老化的功能。 牙骨质的两种主要细胞外成分是无机的。矿物 固体和有机胶原纤维，占80%以上， 组织块 这些成分的化学和结构变化， 在组织成熟过程中发生在分子水平上， 组织功能的质量（即，结构-机械支持）。 目前，在文献中很少有关于 牙骨质中矿物质组成及其与胶原的关系 衰老的迹象 这些属性的描绘，以及老化过程中的变化 在病理条件下，将提供关键信息， 将支持新的临床治疗和材料的开发， 牙周病-一个日益严重的健康问题， 牙科，特别是对越来越多的老年人口。 该研究将广泛利用（1）高压电子 用于化学分析的微衍射/探针微分析;（2） 高压体视显微术和三维计算机序列重建 超微结构表征：（3）用于样品制备，无水 技术和超冷冻显微镜将被采用，以规避 矿物人工制品在制备和分析过程中。 这些技术 代表了当今最强大的工具， 应该产生最亲密的知识牙骨质矿物质和胶原蛋白 还没有得到。