Oats for the future: deciphering potential of host resistance and RNAi to minimise mycotoxin contamination under present and future climate scenarios
未来的燕麦:破译宿主抗性和 RNAi 的潜力,以最大限度地减少当前和未来气候情景下的霉菌毒素污染
基本信息
- 批准号:BB/P001432/1
- 负责人:
- 金额:$ 50.43万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2016
- 资助国家:英国
- 起止时间:2016 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Mycotoxins are natural contaminants of a wide range of staple foods produced by fungi under conducive environmental conditions. The consumption of mycotoxin contaminated foods can result in several acute and chronic diseases in humans and animals. Oats, which have become more popular because if their health benefits can be infected by a Fusarium species, F.langsethiae, which can contaminate oats with the mycotoxins T-2/HT2 toxins. The EU has maximum recommended levels for these two toxins in place. F.langsethiae infects oats during ripening but produces no visible symptoms and is thus difficult to assess except by measuring the mould biomass in the ripening oat grain or by analytically quantifying the amounts of T2/HT-2 present. Limited work has been done on screening oat cultivars for resistance to F.langsethiae infection. Climate change (CC) scenarios has been suggested to probably exacerbate fungal infection and mycotoxin production partially via increases in pest reproduction and damage to ripening crops. Thus there is interest in understanding the relationships between interacting factors of CC including elevated temperature (+2-4oC), x2 or x3 existing CO2 concentrations (400 ppm vs 800 and 1200 ppm CO2) and episodes of drought stress. Very little information is available on how these interacting CC factors impact on infection by this Fusarium species and contamination of oats with the associated mycotoxin. This project is a collaborative project between Cranfield University in the UK and University College Dublin in Ireland to jointly address the issues of a better understanding of the relationship between this important fungal pathogen on different oat cultivars, the impact that CC factors will have on infection and mycotoxin contamination and the development of some novel methods for blocking the production of the mycotoxins.The objectives can be summarised as being (a) In vitro examination of the effect of interacting environmental factors on growth and expression of biosynthetic gene clusters of F.langsethiae during colonisation of oat-based media and oat grains and on T-2/HT-2 toxin production by strains from the UK and Ireland. The effect of interacting CC factors the life cycle of the pathogen will be quantified. RNA-Seq technology will be used to study the gene regulation, focusing on the trichothecenes type A gene cluster for the first time; (b) a range of UK and Heritage Irish oat lines will be screened for distinct gene expression profiles relevant to differential mycotoxin contamination and potential resistance using sequencing approaches; (c) 'In planta' studies using two heritage and UK oat CVs will examine CC factors on plant/fungus gene expression patterns and differential mycotoxin contamination using non-acclimatised and acclimatised strains of F.langsethiae. The effects on shifts/fluctuations in T-2/HT-2 toxin production will be quantified; (d) the relative competitiveness of F.langsethiae and other Fusarium species such as F.graminearum and F.sporotrichioides and oat mycobiota will be examined under normal agronomic and CC conditions in in vitro and in planta studies on dominance at a molecular, colony and ecosystem level; and (e) minimisation strategies using interference RNA technology and novel delivery systems to interfere with the key TRI genes involved in T-2/HT-2 production. In planta trials will be carried out to test control efficacy of toxin production under normal agronomic and CC conditions for the first time.
真菌毒素是由真菌在有利的环境条件下产生的各种主食的天然污染物。食用霉菌毒素污染的食物可导致人类和动物的几种急性和慢性疾病。燕麦,这已经成为更受欢迎的,因为如果他们的健康益处可以感染镰刀菌属物种,F.langsethiae,这可能会污染燕麦与霉菌毒素T-2/HT 2毒素。欧盟对这两种毒素有最高推荐水平。兰瑟镰刀菌在成熟过程中感染燕麦,但不产生可见症状,因此难以评估,除非通过测量成熟燕麦谷粒中的霉菌生物量或通过分析定量存在的T2/HT-2的量。在筛选燕麦品种对F.langsethiae感染的抗性方面所做的工作有限。气候变化(CC)的情况下,可能会加剧真菌感染和真菌毒素的生产,部分通过增加害虫繁殖和损害成熟的作物。因此,人们有兴趣了解气候变化的相互作用因素之间的关系,包括升高的温度(+2-4 ℃),x2或x3现有的CO2浓度(400 ppm与800和1200 ppm CO2)和干旱胁迫事件。关于这些相互作用的CC因子如何影响镰刀菌属物种的感染和相关真菌毒素对燕麦的污染,目前的信息很少。该项目是英国克兰菲尔德大学和爱尔兰的大学学院都柏林之间的合作项目,旨在共同解决更好地了解这种重要的真菌病原体在不同燕麦品种上的关系的问题,CC因子对感染和霉菌毒素污染的影响,以及一些阻断霉菌毒素产生的新方法的发展。(a)在体外检测相互作用的环境因素对长穗镰刀菌在燕麦基培养基和燕麦谷粒定殖期间的生长和生物合成基因簇的表达的影响,以及对来自英国和爱尔兰的菌株产生的T-2/HT-2毒素的影响。将量化相互作用的CC因子对病原体生命周期的影响。(B)将使用测序方法筛选一系列英国和传统爱尔兰燕麦品系的与不同真菌毒素污染和潜在抗性相关的不同基因表达谱;(c)利用两种传统品种和英国燕麦品种进行的“植物内”研究,将研究CC因素对植物/真菌基因表达模式的影响,以及利用非适应和适应的兰瑟氏镰孢菌菌株研究不同的真菌毒素污染。(d)将在正常农艺学和CC条件下,在体外和植物体内对分子、菌落和生态系统水平上的优势进行研究,以检查长穗镰刀菌和其他镰刀菌物种(如禾谷镰刀菌和拟孢镰刀菌)与燕麦真菌生物群的相对竞争力;以及(e)使用干扰RNA技术和新型递送系统干扰参与T-2/HT-2产生的关键TRI基因的最小化策略。将首次进行植物试验,以测试在正常农艺和CC条件下毒素产生的控制功效。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
- DOI:10.1016/j.funbio.2019.11.001
- 发表时间:2021-01-28
- 期刊:
- 影响因子:2.5
- 作者:Cervini, Carla;Verheecke-Vaessen, Carol;Perrone, Giancarlo
- 通讯作者:Perrone, Giancarlo
Growth Kinetic Parameters and Prediction of Growth and Zearalenone and Deoxynivalenol Production Boundaries by Three Fusarium asiaticum Strains Isolated from Wheat
- DOI:10.3390/fermentation8110577
- 发表时间:2022-11-01
- 期刊:
- 影响因子:3.7
- 作者:Garcia-Cela, Esther;Verheecke-Vaessen, Carol;Magan, Naresh
- 通讯作者:Magan, Naresh
Searching for the Fusarium spp. Which Are Responsible for Trichothecene Contamination in Oats Using Metataxonomy to Compare the Distribution of Toxigenic Species in Fields from Spain and the UK.
- DOI:10.3390/toxins14090592
- 发表时间:2022-08-28
- 期刊:
- 影响因子:4.2
- 作者:Gil-Serna J;Patiño B;Verheecke-Vaessen C;Vázquez C;Medina Á
- 通讯作者:Medina Á
Acclimatisation of Fusarium langsethiae, F. poae and F. sporotrichioides to elevated CO2: Impact on fungal growth and mycotoxin production on oat-based media
Fusarium langsethiae、F. poae 和 F. sporotrichioides 对升高的 CO2 的适应:对燕麦培养基上真菌生长和霉菌毒素产生的影响
- DOI:10.1016/j.ijfoodmicro.2023.110176
- 发表时间:2023
- 期刊:
- 影响因子:5.4
- 作者:Kahla A
- 通讯作者:Kahla A
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Naresh Magan其他文献
Impact of Water Activity and Temperature on Growth and Ochratoxin A Production of Two <em>Aspergillus carbonarius</em> Isolates from Wine Grapes in Greece
- DOI:
10.4315/0362-028x-70.12.2884 - 发表时间:
2007-12-01 - 期刊:
- 影响因子:
- 作者:
Chrysoula C. Tassou;Pantelis I. Natskoulis;Efstathios Z. Panagou;Apostolos E. Spiropoulos;Naresh Magan - 通讯作者:
Naresh Magan
Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation: effect of water activity on accumulation of compatible solutes
- DOI:
10.1023/a:1007082829307 - 发表时间:
1999-05-01 - 期刊:
- 影响因子:2.900
- 作者:
Susana Pascual;Paloma Melgarejo;Naresh Magan - 通讯作者:
Naresh Magan
The impact of water and temperature interactions on lag phase, growth and potential ochratoxin A production by two new species, <em>Aspergillus aculeatinus</em> and <em>A</em>. <em>sclerotiicarbonarius</em>, on a green coffee-based medium
- DOI:
10.1016/j.ijfoodmicro.2014.07.025 - 发表时间:
2014-10-01 - 期刊:
- 影响因子:
- 作者:
Asya Akbar;Naresh Magan - 通讯作者:
Naresh Magan
Comparison of growth and aflatoxin Bsub1/sub production profiles of emAspergillus flavus/em strains on conventional and isogenic GM-maize-based nutritional matrices
- DOI:
10.1016/j.funbio.2021.10.006 - 发表时间:
2022-01-01 - 期刊:
- 影响因子:3.000
- 作者:
Alessandra M. Gasperini;Angel Medina;Naresh Magan - 通讯作者:
Naresh Magan
Efficacy of sodium metabisulphite for control of emAspergillus flavus/em and aflatoxin Bsub1/sub contamination emin vitro/em and in chilli powder and whole red chillies
- DOI:
10.1016/j.foodcont.2021.108786 - 发表时间:
2022-05-01 - 期刊:
- 影响因子:6.300
- 作者:
Diyaa Al-Jaza;Angel Medina;Naresh Magan - 通讯作者:
Naresh Magan
Naresh Magan的其他文献
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