TRANSVERSE NOE IN BIOMACROMOLECULES

自旋锁定态中的偶极弛豫现象 将探索复杂生物分子中的自旋1/2核。克罗斯- 松弛过程将催生一家Overhauser 对阐明结构和结构有用的效应 生物分子在溶液中的构象。我们将尝试 产生纯平稳锁定的设计脉冲序列 态，从而干涉双量子和哈特曼-哈恩 效果被抑制。将使用密度矩阵方法来 分析复杂自旋系统在不同条件下的行为 外加R.F.的同时影响。锁定字段和 磁偶极弛豫。所有的理论预测和 协议将使用刚性模型进行实验检查 已知结构的化合物。到那时，开发的方法将 在生物分子构象研究中的应用 解决方案。

The phenomenon of dipolar relaxation in spin-locked states of spin-1/2 nuclei in complex biomolecules will be explored. Cross- relaxation processes will give rise to a family of Overhauser effects which can be useful in the elucidation of structure and conformation of biomolecules in solutions. We will attempt to design pulse sequences giving rise to pure stationary locked states, so that interfering double-quantum and Hartmann-Hahn effects are suppressed. Density matrix methods will be used to analyze the behavior of complex spin systems under the simultaneous influence of an applied r.f. locking field and magnetic dipolar relaxation. All theoretical predications and protocols will be checked experimentally using rigid model compounds of known structures. The methods developed will then be applied to the investigation of conformation of biomolecules in solution.