TRANSVERSE NOE IN BIOMACROMOLECULES

生物大分子中的横向 NOE

• 批准号: 3225587
• 负责人: AKSEL A BOTHNER-BY
• 金额: $ 15.09万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225587
• 关键词: chemical structure; clinical biomedical equipment; conformation; electron density; mathematical model; neurophysins; nuclear magnetic resonance spectroscopy; solute

The phenomenon of dipolar relaxation in spin-locked states of spin-1/2 nuclei in complex biomolecules will be explored. Cross- relaxation processes will give rise to a family of Overhauser effects which can be useful in the elucidation of structure and conformation of biomolecules in solutions. We will attempt to design pulse sequences giving rise to pure stationary locked states, so that interfering double-quantum and Hartmann-Hahn effects are suppressed. Density matrix methods will be used to analyze the behavior of complex spin systems under the simultaneous influence of an applied r.f. locking field and magnetic dipolar relaxation. All theoretical predications and protocols will be checked experimentally using rigid model compounds of known structures. The methods developed will then be applied to the investigation of conformation of biomolecules in solution.

的自旋锁定态中的偶极弛豫现象