EXPRESSION OF PREPROENKEPHALIN-LIKE MRNA IN THE TESTIS

最近的研究表明，阿片肽存在于 睾丸，似乎作为旁分泌或自分泌调节剂， 睾丸功能 首席研究员现在已经获得了新的 阿片肽前体mRNA表达的证据， 前脑啡肽，在大鼠睾丸。 这个睾丸的大小 前脑啡肽原mRNA是独特的， 前脑啡肽原mRNA的任何组织先前检查。 本提案的具体目标是：（1）识别细胞类型 表达前脑啡肽原样mRNA和前脑啡肽原衍生肽 在大鼠睾丸中;（2）表征衍生的初级翻译产物 从睾丸前脑啡肽原样mRNA;（3）确定核苷酸 大鼠睾丸前脑啡肽原样mRNA的序列;（4）表征 睾丸前脑啡肽原样肽产物 蛋白 这些研究将确定前脑啡肽原mRNA的性质， 蛋白质在大鼠睾丸，将是必不可少的未来预测 研究细胞的作用部位和激素 脑啡肽原基因表达的调节。

Recent studies have indicated that opioid peptides are present within the testis and appear to serve as paracrine or autocrine regulators of testicular function. The principal investigator has now obtained new evidence for the expression of an mRNA for the opioid peptide precursor, proenkephalin, in the rat testis. The size of this testicular preproenkephalin mRNA is unique, being substantially larger than preproenkephalin mRNAs from any tissue previously examined. The specific aims of this proposal are to: (1) identify the cell types that express preproenkephalin-like mRNA and proenkephalin-derived peptides in rat testis; (2) characterize the primary translation product derived from testicular preproenkephalin-like mRNA; (3) determine the nucleotide sequence of preproenkephalin-like mRNA in the rat testis; (4) characterize the peptide products derived from the testicular preproenkephalin-like protein. These studies will define the nature of preproenkephalin mRNA and protein in the rat testis and will be essential for future projected investigations into the cellular sites of action and the hormonal regulation of proenkephalin gene expression in this tissue.